SEC31A Antibody (5D12C6) - BSA Free

Western Blot: SEC31A Antibody (5D12C6) [NBP2-61870] - Analysis using SEC31A mAb against human SEC31A (AA: 429-571) recombinant protein. (Expected MW is 41.8 kDa)

Immunohistochemistry: SEC31A Antibody (5D12C6) [NBP2-61870] - Analysis of paraffin-embedded ovarian cancer tissues using SEC31A mouse mAb with DAB staining.

Western Blot: SEC31A Antibody (5D12C6) [NBP2-61870] - Analysis using SEC31A mAb against HEK293 (1) and SEC31A (AA: 429-571)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: SEC31A Antibody (5D12C6) [NBP2-61870] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 5D12C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SEC31A Antibody (5D12C6) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human SEC31A (AA: 429-571) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SEC31A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 133 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for SEC31A Antibody (5D12C6) - BSA Free

• ABP125ABP130KIAA0905Web1-like protein
• DKFZp686N07171
• HSPC334
• MGC90305
• protein transport protein Sec31A
• protein-transport protein SEC31
• SEC31 homolog A (S. cerevisiae)
• SEC31L1
• SEC31-like 1 (S. cerevisiae)
• SEC31-like 1
• SEC31-like protein 1
• SEC31-related protein A
• yeast Sec31p homolog

Background

The protein encoded by this gene shares similarity with the yeast Sec31 protein, and is a component of the outer layer of the coat protein complex II (COPII). The encoded protein is involved in vesicle budding from the endoplasmic reticulum (ER) and contains multiple WD repeats near the N-terminus and a proline-rich region in the C-terminal half. It associates with the protein encoded by the SEC13 homolog, nuclear pore and COPII coat complex component (SEC13), and is required for ER-Golgi transport. Monoubiquitylation of this protein by CUL3-KLHL12 was found to regulate the size of COPII coats to accommodate unusually shaped cargo. Alternative splicing results in multiple transcript variants encoding different isoforms.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEC31A