SEC23A Antibody (JU37-48)

Western Blot: SEC23A Antibody (JU37-48) [NBP2-75428] - Analysis of SEC23 on NIH-3T3 cell using anti-SEC23 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: SEC23A Antibody (JU37-48) [NBP2-75428] - Staining SEC23 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SEC23A Antibody (JU37-48) [NBP2-75428] - Analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-SEC23 antibody. Counter stained with hematoxylin.

Flow Cytometry: SEC23A Antibody (JU37-48) [NBP2-75428] - Analysis of HepG2 cells with SEC23 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: SEC23A Antibody (JU37-48) [NBP2-75428] - Staining SEC23 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SEC23A Antibody (JU37-48) [NBP2-75428] - Analysis of paraffin-embedded human tonsil tissue using anti-SEC23 antibody. Counter stained with hematoxylin.

Product Discontinued

SEC23A Antibody (JU37-48) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human SEC23A aa 280-420 / 765. (SwissProt: Q15436 Human; SwissProt: Q01405 Mouse)
• Localization: Cytosol. Endoplasmic reticulum.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SEC23A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SEC23A Antibody (JU37-48)

• CLSD
• MGC26267
• protein transport protein Sec23A
• Sec23 (S. cerevisiae) homolog A
• Sec23 homolog A (S. cerevisiae)
• SEC23-related protein A

Background

SEC23A is encoded by this gene is a member of the SEC23 subfamily of the SEC23/SEC24 family. It is part of a protein complex and found in the ribosome-free transitional face of the endoplasmic reticulum (ER) and associated vesicles. This protein has similarity to yeast Sec23p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The encoded protein is suggested to play a role in the ER-Golgi protein trafficking. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEC23A