SDS3 Antibody - BSA Free

Western Blot: SDS3 Antibody [NB100-257] - Detection of Human and Mouse SDS3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-SDS3 antibody NB100-257 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: SDS3 Antibody [NB100-257] - Detection of human SDS3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-SDS3 antibody NB100-257 (lot 2) used for IP at 6 ug per reaction. SDS3 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-SDS3 antibody NB100-93318. For blotting immunoprecipitated SDS3, NB100-257 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu, Bv, Pm
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SDS3 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 175 and 225 of human Suppressor of Defective Silencing 3 using the numbering given in entry NP_071936.2 (GeneID 64426).
• Predicted Species: Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SUDS3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:1000-1:5000
• Application Notes: By Western blot, this antibody recognizes bands around 45 kDa representing SDS3.
• Control:

  HeLa Whole Cell Lysate
  SDS3 Overexpression Lysate

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SDS3 Antibody - BSA Free

• 45 kDa Sin3-associated polypeptide
• FLJ00052
• SAP45suppressor of defective silencing 3 homolog (SDS3, S. cerevisiae)
• SDS3MGC104711
• sin3 histone deacetylase corepressor complex component SDS3
• sin3A-associated protein, 45kDa
• suppressor of defective silencing 3 homolog (S. cerevisiae)
• Suppressor of defective silencing 3 protein homolog

Background

SDS3 is a subunit of the histone deacetylase-dependent SIN3A corepressor complex. The SIN3A corepressor complex functions to repress transcription through its interaction with histone deacetylases HDAC1 and HDAC2. SDS3 has been demonstrated to play a role in pericentric heterochromatin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SDS3 Antibody (NB100-257)(3)

Publications using NB100-257

• Winter SF, Lukes L, Walker RC et al. Allelic variation and differential expression of the mSIN3A histone deacetylase complex gene Arid4b promote mammary tumor growth and metastasis. PLoS Genet 2012-05-01 [PMID: 22693453]
• Hurst DR, Mehta A, Moore BP et al. Breast cancer metastasis suppressor 1 (BRMS1) is stabilized by the Hsp90 chaperone. Biochem Biophys Res Commun 2006-10-01 [PMID: 16919237]
• Li Z, David G, Hung KW et al. Cdk5/p35 phosphorylates mSds3 and regulates mSds3-mediated repression of transcription. J Biol Chem 2004-12-01 [PMID: 15489224]

Bioinformatics

• Gene Symbol: SUDS3
• Entrez:
  • 64426(Human)
  • 71954(Mouse)
• Uniprot:
  • NP_071936.2(Human)