SDHA Antibody

Images

 
Western Blot: SDHA Antibody [NBP1-71688] - Analysis of SDHA in NIH/3T3 cells.
Immunocytochemistry/ Immunofluorescence: SDHA Antibody [NBP1-71688] - SDHA antibody was tested at 1:100 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).
Western Blot: SDHA Antibody [NBP1-71688] - analysis of SDHA in whole skeletal muscle lysate or purified mitochondria from pig, mouse, lamb and goat using anti-SDHA antibody. Image from verified customer review.
Simple Western: SDHA Antibody [NBP1-71688] - Simple Western lane view shows a specific band for SDHA in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

SDHA Antibody Summary

Immunogen
A genomic peptide made to a C-terminal region of the human SDHA protein (within residues 500-664). [Swiss-Prot P31040]
Localization
Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Predicted Species
Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SDHA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
Application Notes
This SDHA antibody is useful for ICC/IF and Western blot, where a band is seen ~72 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
NIH 3T3 Lysate (NB800-PC8)
Reviewed Applications
Read 4 Reviews rated 4.5
using
NBP1-71688 in the following applications:

Publications
Read Publication using
NBP1-71688 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human and mouse. Porcine reactivity reported by a customer review. Immunogen has 94% identity to rat and 93% identity to porcine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.1% BSA, and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for SDHA Antibody

  • CMD1GG
  • EC 1.3.5.1
  • Fp
  • SDH1
  • SDH2
  • SDHFmitochondrial
  • succinate dehydrogenase complex flavoprotein subunit
  • succinate dehydrogenase complex, subunit A, flavoprotein (Fp)

Background

SDHA is an important component in the citric acid cycle and respiratory chain. SDHA converts succinate to fumarate, transfers electrons in the respiratory chain, and ultimately transfers electrons to ubiquinone. SDHA is localized within the mitochondrial inner membrane. Mutations in the gene have been associated with Leigh syndrome and Huntington's disease. However, SDHA also appears to have tumor suppressor effects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SDHA Antibody (NBP1-71688)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for SDHA Antibody (NBP1-71688) (4) 4.54

Average Rating: 4.5
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Other.
We have 4 reviews tested in 2 application: Simple Western, WB.

Reviews using NBP1-71688:
Filter by Applications
Simple Western
(1)
WB
(3)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Other
(2)
All Species
Images Ratings Applications Species Date Details
Simple Western SDHA NBP1-71688
Enlarge
reviewed by:
Anonymous
Simple Western Mouse 12/30/2015
View

Summary

ApplicationSimple Western
Sample TestedMouse whole liver
SpeciesMouse

Blocking

Blocking DetailsDefault settings of ProteinSimple Simple Western assay

Primary Anitbody

Dilution Ratio1:100 and Default settings of ProteinSimple Simple Western assay

Secondary Antibody

Secondary DescriptionGoat anti-rabbit HRP
Secondary Manufacturer Cat#042-206

Details

Detection NotesProtein Simple WES system

Comments

CommentsProtein concentration of 1.5 ug/ul
Western Blot SDHA NBP1-71688
Enlarge
reviewed by:
Eric England
WB Other 11/03/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole Skeletal Muscle Lysate or Purified Mitochondria
SpeciesOther
LotA-1
CommentsLane Key:1: Whole Porcine Skeletal Muscle Lysate2: Purified Mouse Mitochondria3: Whole Mousee Skeletal Muscle Lysate4: Whole Goat Skeletal Muscle Lysate5: Whole Lamb Skeletal Muscle Lysate

Blocking

Blocking Details3% Casein in PBS with 0.1% Tween-20 for 1 hr

Primary Anitbody

Dilution Ratio1/2000 at 4C for 18 hr

Secondary Antibody

Secondary DescriptionDonkey anti-Rabbit IRDye 800CW - Licor Biosciences
Secondary Manufacturer Cat#926-32213
Secondary Concentration1/25,000

Details

Detection NotesLicor Odyssey

Comments

CommentsLane Key:1: Whole Porcine Skeletal Muscle Lysate2: Purified Mouse Mitochondria3: Whole Mousee Skeletal Muscle Lysate4: Whole Goat Skeletal Muscle Lysate5: Whole Lamb Skeletal Muscle Lysate
Western Blot SDHA NBP1-71688
Enlarge
reviewed by:
Tracy Scheffler
WB Other 10/27/2011
View

Summary

ApplicationWestern Blot
Sample TestedPig skeletal muscle extract, Sample Amount: 10-30 ug
SpeciesOther
LotA1
CommentsPig skeletal muscle extract

Blocking

Blocking DetailsBlocking Buffer: Thermo TBS starting blocking buffer, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer: Blocking buffer + 0.05% tween, Incubation Time: 2hr, Incubation Temp:RT

Secondary Antibody

Secondary DescriptionSecondary Ab: IRDye Donkey anti-rabbit 80, Secondary Ab Dilution Ratio: 1:10,000

Details

Detection NotesDetection Method: Odyssey infrared imager, Specific Bands: 72 kDa

Comments

CommentsPig skeletal muscle extract
Western Blot SDHA NBP1-71688
Enlarge
reviewed by:
Hongxia Hu
WB Human 10/01/2011
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman

Product General Protocols

View specific protocols for SDHA Antibody (NBP1-71688): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SDHA Antibody (NBP1-71688) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional SDHA Products

Bioinformatics Tool for SDHA Antibody (NBP1-71688)

Discover related pathways, diseases and genes to SDHA Antibody (NBP1-71688). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SDHA Antibody (NBP1-71688)

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Pathways for SDHA Antibody (NBP1-71688)

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PTMs for SDHA Antibody (NBP1-71688)

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Research Areas for SDHA Antibody (NBP1-71688)

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Blogs on SDHA.

SDHA - oxidative enzyme in the citric acid cycle
Succinate dehydrogenase is an important tetrameric protein involved in the citric acid cycle. It is localized to the inner mitochondrial membrane of cells. Succinate dehydrogenase makes up Complex II of the electron transport chain (ETC) and is re...  Read full blog post.

SDHA - An essential Krebs cycle enzyme with role in cancer and metabolism
Succinate dehydrogenase (SDH) is a highly conserved protein complex located on the inner mitochondrial membrane where it functions during the Krebs cycle by oxidizing succinate to fumarate (1). This reaction is also important for feeding electrons...  Read full blog post.

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Recent Reviews

4.5
5
2
4
2
3
0
2
0
1
0

Anonymous
12/30/2015
Application: Simple Western
Species: Mouse

Eric England
11/03/2014
Application: WB
Species: Other

Tracy Scheffler
10/27/2011
Application: WB
Species: Other

Bioinformatics

Gene Symbol SDHA
Entrez
Uniprot