Scribble Antibody (7C6.D10) - BSA Free

Western Blot: Scribble Antibody (7C6.D10) - BSA Free [NBP2-29765] - Hela cell lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Scribble, clone 7C6.D10 (0.1 ug/mL at 1 mg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP (AP124P) and a chemiluminescence detection system. Arrow indicates Scribble (~220 kDa).

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 7C6.D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Scribble Antibody (7C6.D10) - BSA Free Summary

• Description: Relevant Entrez Gene Numbers: AAL38976
• Immunogen: GST-tagged fusion protein corresponding to the PDZ2 domain of Scribble.
• Specificity: PDZ2 domain of Scribble.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SCRIB
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
• Theoretical MW: 220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to cross react with mouse based on sequence homology. Reactivity with other species has not been tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Notes

Relevant Entrez Gene Numbers: AAL38976

Alternate Names for Scribble Antibody (7C6.D10) - BSA Free

• protein scribble homolog
• CRIB1
• SCRB1
• SCRIB1
• scribbled homolog (Drosophila)
• Vartul

Background

Scribble is a tumor suppressor marker first discovered in Drosophila that has been implicated in the control of cell proliferation, polarity, invasion, and metastasis (Humbert, et al, 2003). Mammalian Scribble expresses basolateral staining in epithelial tissue and highly polarized expression in T lymphocytes (Dow, et al, 2003) (Ludford-Menting, et al, 2005), and plays an essential role in the regulation of the polarity specifically involved in directed epithelial migration. Altered expression of human Scribble is associated with invasive epithelial cancers, however, its role in tumour development remains unclear (Dow, et al, 2007).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCRIB
• Uniprot:
  • Q14160(Human)