SCNN1D Antibody

Immunohistochemistry-Paraffin: SCNN1D Antibody [NBP1-45221] - (2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SCNN1D Antibody Summary

• Description: Novus Biologicals Goat SCNN1D Antibody (NBP1-45221) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-SCNN1D Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-AGPQPLETLDT corresponding to C-Terminus according to NP_002969.2.
• Epitope: C-Terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SCNN1D
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 ug/ml
• Application Notes: Western blot: Preliminary experiments in Human Pancreas and Thymus lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
  IHC: Paraffin embedded Human Uterus.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SCNN1D Antibody

• Delta-ENaC
• Delta-NaCH
• DNACH
• ENaCD
• MGC149710
• MGC149711
• SCNED
• SCNN1D
• sodium channel, nonvoltage-gated 1, delta
• voltage-gated, type I, delta polypeptide

Background

SCNN1D is a subunit of the epithelial sodium channel (ENaC). ENaC has high sodium selectivity, low conductance, and amiloride sensitivity. The epithelial Na(+) channel (ENaC) regulates Na(+) homeostasis in cells and across epithelia; in the kidney, lung and colon it plays an essential role in trans-epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium re-uptake in the distal nephron of the kidney, thus regulating blood pressure. Four homologous ENaC subunits (alpha, beta, gamma, and delta) have been isolated in mammals. Combination of alpha-, beta-, and gamma-subunits or delta-, beta-, and gamma-subunits forms fully functional channels. A delta subunit can replace the alpha subunit. However, the pharmacology, sensitivity to amiloride, conductance, and ionic selectivity of the delta/beta-gamma channel are different from those of the alpha/beta-gamma channel. FUNCTION: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. SUBUNIT: Heterotetramer of two alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SCNN1D Antibody (NBP1-45221)(1)

Publication using NBP1-45221

• Giraldez T, Afonso-Oramas D, Cruz-Muros I et al. Cloning and functional expression of a new epithelial sodium channel delta subunit isoform differentially expressed in neurons of the human and monkey telencephalon J Neurochem 2007-08-01 [PMID: 17472699]

Bioinformatics

• Gene Symbol: SCNN1D
• Entrez:
  • 6339(Human)