Scn2b Antibody (S395-68)

Western Blot: Scn2b Antibody (S395-68) [NBP2-59317] - Analysis of Rat Brain showing detection of ~40 kDa Nav-Beta-2 protein using Mouse Anti-Nav-Beta-2 Monoclonal Antibody, Clone S395-68. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 ug . Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Nav-Beta-2 Monoclonal Antibody at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~40 kDa.

Immunocytochemistry/Immunofluorescence: Scn2b Antibody (S395-68) [NBP2-59317] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Nav beta 2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X.

Product Discontinued

Scn2b Antibody (S395-68) Summary

• Immunogen: Fusion protein amino acids 1-215 (full-length) of mouse Nav beta2
• Localization: Membrane
• Specificity: Detects 40kDa. Does not cross-reat with NavBeta1, Navbeta3, or Navbeta4.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SCN2B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:100 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Purity: Protein G purified

Alternate Names for Scn2b Antibody (S395-68)

• neuronal voltage-gated sodium channel beta 2 subunit
• sodium channel beta 2 subunit
• sodium channel subunit beta-2
• sodium channel, voltage-gated, type II, beta polypeptide
• sodium channel, voltage-gated, type II, beta

Background

Voltage-gated sodium channels (NaV) are responsible for action potential initiation and propagation in excitable cells, including nerve, muscle, and neuroendocrine cell types. They are also expressed at low levels in non-excitable cells, where their physiological role is unclear. Structurally, Nav channels are composed of one pore-forming alpha-subunit, which may be associated with either one or more beta-subunits. alpha-subunits are composed for four homologous domains, each of which contains six transmembrane segments. The fourth transmembrane loop (S4) acts as the 'voltage sensor' and is activated by changes in membrane potential. S4 is also involved in channel gating.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCN2B