SCARA3 Overexpression Lysate (Native)

Images

 
Western Blot: SCARA3 Overexpression Lysate (Adult Normal) [NBL1-15719] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SCARA3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SCARA3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209235 and based on accession number NM_182826. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens scavenger receptor class A, member 3 (SCARA3), transcript variant 2, mRNA.
Gene
SCARA3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
52.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SCARA3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SCARA3 Overexpression Lysate (Native)

  • APC7
  • CSR1
  • CSRCellular stress response gene protein
  • macrophage scavenger receptor-like 1
  • MSLR1cellular stress response protein
  • MSRL1
  • scavenger receptor class A member 3
  • scavenger receptor class A, member 3

Background

This gene encodes a macrophage scavenger receptor-like protein. This protein has been shown to deplete reactive oxygen species, and thus play an important role in protecting cells from oxidative stress. The expression of this gene is induced by oxidative stress. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]. Transcript Variant: This variant (2), also known as CSR2, differs in the 3' end-region, which includes a part of the coding region, as compared to variant 1. The resulting isform (2) has a distinct and shorter C-terminus, as compared to isoform 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-30955
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-57516
Species: Hu
Applications: WB
NBP2-39019
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
DDX0131P-100
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
AF5745
Species: Hu
Applications: WB, IHC
AF4885
Species: Mu
Applications: WB, IP
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF6387
Species: Hu
Applications: WB
NB100-56516
Species: Hu, Mu
Applications: WB, IHC
NBP1-97587
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
NBP1-89095
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB600-1031
Species: Hu, Mu, Rt, Rb
Applications: WB, ICC/IF, IHC-P
NB600-235
Species: Hu, Mu, Po
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF8221
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC
NBP2-21037
Species: Hu
Applications: WB, IHC, IHC-P
NBL1-15719
Species: Hu
Applications: WB

Publications for SCARA3 Lysate (NBL1-15719) (0)

There are no publications for SCARA3 Lysate (NBL1-15719).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SCARA3 Lysate (NBL1-15719) (0)

There are no reviews for SCARA3 Lysate (NBL1-15719). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SCARA3 Lysate (NBL1-15719) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SCARA3 Products

Bioinformatics Tool for SCARA3 Lysate (NBL1-15719)

Discover related pathways, diseases and genes to SCARA3 Lysate (NBL1-15719). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SCARA3 Lysate (NBL1-15719)

Discover more about diseases related to SCARA3 Lysate (NBL1-15719).
 

Pathways for SCARA3 Lysate (NBL1-15719)

View related products by pathway.

PTMs for SCARA3 Lysate (NBL1-15719)

Learn more about PTMs related to SCARA3 Lysate (NBL1-15719).

Blogs on SCARA3

There are no specific blogs for SCARA3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-4 [Unconjugated]
204-IL-010/CF
SCARA3 Antibody
NBP2-13286

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SCARA3 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol SCARA3