Recombinant Human SAR1 Protein

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SDS-Page: SAR1 Protein [NBP1-30177] - SAR1A, 24.5 kDa (218aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human SAR1 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1- 198 of Human SAR1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSFIFEWIYN GFSSVLQFLG LYKKSGKLVF LGLDNAGKTT LLHMLKDDRL GQHVPTLHPT SEELTIAGMT FTTFDLGGHE QARRVWKNYL PAINGIVFLV DCADHSRLVE SKVELNALMT DETISNVPIL ILGNKIDRTD AISEEKLREI FGLYGQTTGK GNVTLKELNA RPMEVFMCSV LKRQGYGEGF RWLSQYID

Protein/Peptide Type
Recombinant Protein
Gene
SAR1A
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
24.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human SAR1 Protein

  • COPII-associated small GTPase
  • EC 3.6.5
  • masra2
  • SAR1 gene homolog A
  • SAR1 homolog A (S. cerevisiae)
  • SAR1a gene homolog (S. cerevisiae) 1
  • SAR1a gene homolog 1 (S. cerevisiae)
  • SAR1a gene homolog 1
  • SAR1SARA1GTP-binding protein SAR1a
  • Sara

Background

SAR1a gene homolog 1, also known as SAR1A, belongs to the small GTPase superfamily. Sar1 is an essential component of COPII vesicle coats involved in export of cargo from the ER. The GTPase activity of Sar1 functions as a molecular switch to control protein-protein and protein-lipid interactions that direct vesicle budding from the ER. GDP-bound Sar1 interacts with the membrane-bound exchange factor Sec12 and exchanges its bound GDP for GTP. Recombinant human SAR1A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol SAR1A