![Western Blot: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of SF3B1 on different lysates using anti-SF3B1 antibody at 1/500 dilution.Positive control:Lane 1: Mouse thymus Lane 2: Hela](http://images.novusbio.com/fullsize2/SAP155-Antibody-(JB40-32)-Western-Blot-NBP2-75656-img0006.jpg "Western Blot: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of SF3B1 on different lysates using anti-SF3B1 antibody at 1/500 dilution.Positive control:Lane 1: Mouse thymus Lane 2: Hela")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC-P |
| Clone | JB40-32 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant protein within N-terminal Human SAP155 . (SwissProt: O75533 Human;SwissProt: Q99NB9 Mouse) |
| Localization | Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SF3B1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 146 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 1% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Immunohistochemistry-Paraffin: FANCB Antibody (M38P3E10) [NBP2-52406] - Staining was performed on formalin-fixed, paraffin-embedded human breast cancer tissue sections using anti-FANCB [M38P3E10]. The antibody shows both nuclear and cytoplasmic staining but it is predominately cytoplasmic.](http://images.novusbio.com/images2/FANCB-Antibody-(M38P3E10)-Immunohistochemistry-Paraffin-NBP2-52406-img0001.jpg)
![Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - An intracellular stain was performed on Raw264.7 cells with CD34 Antibody [MEC 14.7] NB600-1071 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488 (SA5-10018, Thermo Fisher).](http://images.novusbio.com/images2/CD34-Antibody-(MEC-14.7)-Flow-Cytometry-NB600-1071-img0015.jpg)
![Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).](http://images.novusbio.com/images2/CD34-Antibody-(MEC-14.7)-Flow-Cytometry-NB600-1071-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/images2/CD34-Antibody-(MEC-14.7)-Immunocytochemistry-Immunofluorescence-NB600-1071-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MID1 Antibody [NBP3-05061] - Analysis of MCF-7 cells using MID1 antibody . Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/MID1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05061-img0001.jpg)
![Western Blot: MID1 Antibody [NBP3-05061] - analysis of extracts of HeLa cells, using MID1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/images2/MID1-Antibody-Western-Blot-NBP3-05061-img0003.jpg)
![Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Flow-Cytometry-NB200-103-img0006.jpg)
![Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Flow-Cytometry-NB200-103-img0007.jpg)
![Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Flow-(Intracellular)-NB200-103-img0003.jpg)
![Protein Array: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HLA DQ/DR/DP Antibody (HLA-Pan/2967R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Protein-Array-NBP2-79843-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Immunofluorescence staining of Raji cells using HLA DQ/DR/DP Antibody (HLA-Pan/2967R) followed by goat anti-rabbit IgG-CF488 (Green). Nuclei stained with RedDot (Red).](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-79843-img0002.jpg)
![Western Blot: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Western Blot Analysis of Ramos cell lysate using HLA DQ/DR/DP Antibody (HLA-Pan/2967R).](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Western-Blot-NBP2-79843-img0003.jpg)
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Flow-Cytometry-NB100-524-img0014.jpg)
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Flow-Cytometry-NB100-524-img0015.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Flow Cytometry: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by CDKN2B antibody.](http://images.novusbio.com/images2/p15INK4b-CDKN2B-Antibody-(OTI3B6)-Flow-Cytometry-NBP2-45603-img0001.jpg)
![Immunohistochemistry: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of Human pancreas tissue.](http://images.novusbio.com/images2/p15INK4b-CDKN2B-Antibody-(OTI3B6)-Immunohistochemistry-NBP2-45603-img0002.jpg)
![Immunohistochemistry: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/images2/p15INK4b-CDKN2B-Antibody-(OTI3B6)-Immunohistochemistry-NBP2-45603-img0003.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for SAP155 Antibody (NBP2-75656)Discover more about diseases related to SAP155 Antibody (NBP2-75656).
| Pathways for SAP155 Antibody (NBP2-75656)View related products by pathway.
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PTMs for SAP155 Antibody (NBP2-75656)Learn more about PTMs related to SAP155 Antibody (NBP2-75656).
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| Gene Symbol | SF3B1 |
![Immunohistochemistry-Paraffin: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of paraffin-embedded rat brain tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/SAP155-Antibody-(JB40-32)-Immunohistochemistry-Paraffin-NBP2-75656-img0005.jpg "Immunohistochemistry-Paraffin: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of paraffin-embedded rat brain tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of paraffin-embedded human colon cancer tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/SAP155-Antibody-(JB40-32)-Immunohistochemistry-Paraffin-NBP2-75656-img0001.jpg "Immunohistochemistry-Paraffin: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of paraffin-embedded human colon cancer tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of paraffin-embedded human skin tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/SAP155-Antibody-(JB40-32)-Immunohistochemistry-Paraffin-NBP2-75656-img0002.jpg "Immunohistochemistry-Paraffin: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of paraffin-embedded human skin tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of paraffin-embedded human kidney tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/SAP155-Antibody-(JB40-32)-Immunohistochemistry-Paraffin-NBP2-75656-img0003.jpg "Immunohistochemistry-Paraffin: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of paraffin-embedded human kidney tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of paraffin-embedded mouse small intestine tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/SAP155-Antibody-(JB40-32)-Immunohistochemistry-Paraffin-NBP2-75656-img0004.jpg "Immunohistochemistry-Paraffin: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of paraffin-embedded mouse small intestine tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.")
![Immunocytochemistry/Immunofluorescence: RUNX1/CBFA2 Antibody [NBP1-89105] - Staining human cell line A-431 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/images2/RUNX1-CBFA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89105-img0008.jpg)
![Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody [NBP1-89105] - Staining of human breast shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/images2/RUNX1-CBFA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89105-img0013.jpg)
![Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody [NBP1-89105] - Staining of human Fallopian tube shows weak to moderate nuclear positivity in glandular cells.](http://images.novusbio.com/images2/RUNX1-CBFA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89105-img0014.jpg)
![Immunohistochemistry-Paraffin: EPX Antibody [NBP2-13967] - Staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/images2/EPX-Antibody-Immunohistochemistry-Paraffin-NBP2-13967-img0002.jpg)
![Immunohistochemistry-Paraffin: EPX Antibody [NBP2-13967] - Staining of human duodenum shows moderate cytoplasmic positivity in some peripheral leukocytes.](http://images.novusbio.com/images2/EPX-Antibody-Immunohistochemistry-Paraffin-NBP2-13967-img0003.jpg)
![Immunohistochemistry-Paraffin: EPX Antibody [NBP2-13967] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/images2/EPX-Antibody-Immunohistochemistry-Paraffin-NBP2-13967-img0004.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")