SAP155 Antibody (JB40-32)

Western Blot: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of SF3B1 on different lysates using anti-SF3B1 antibody at 1/500 dilution.Positive control:Lane 1: Mouse thymus Lane 2: Hela

Immunohistochemistry-Paraffin: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of paraffin-embedded rat brain tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of paraffin-embedded human colon cancer tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of paraffin-embedded human skin tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of paraffin-embedded human kidney tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SAP155 Antibody (JB40-32) [NBP2-75656] - Analysis of paraffin-embedded mouse small intestine tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.

Product Discontinued

SAP155 Antibody (JB40-32) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human SAP155 aa 1-105 / 1,304. (SwissProt: O75533 Human; SwissProt: Q99NB9 Mouse)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SF3B1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 146 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SAP155 Antibody (JB40-32)

• Hsh155
• pre-mRNA processing 10
• pre-mRNA splicing factor SF3b, 155 kDa subunit
• Pre-mRNA-splicing factor SF3b 155 kDa subunit
• Prp10
• PRPF10
• SAP 155
• SAP155splicing factor 3b, subunit 1, 155kD
• SF3B155
• SF3b155PRP10
• spliceosome associated protein 155
• Spliceosome-associated protein 155
• splicing factor 3B subunit 1
• splicing factor 3b, subunit 1, 155kDa

Background

SF3B155 is a subunit of the U2 small nuclear ribonucleoproteins complex (U2 snRNP) that functions in pre-mRNA splicing. SF3B155 together with splicing factor 3a binds pre-mRNA upstream of the branchpoint sequence to anchor U2 snRNP to the pre-mRNA. SF3B155 is also a component of the U12-type splicesome that is involved in the splicing of a rare class of nuclear pre-mRNA introns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SF3B1