SAMSN1 Antibody

Western Blot: SAMSN1 Antibody [NB300-976] - (1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SAMSN1 Antibody Summary

• Immunogen: Peptide with sequence IRLDTLSASLGRSC corresponding to N-Terminus according to NP_071419.2.
• Specificity: Please note that the immunogen sequence is present in NP_071419.2 (and in AAK07746) but is no longer present in the latest reference sequence (NP_071419.3 at 05/10/05). This latest version has not been subject to final review by NCBI. This antibody may be recognising one particular isoform.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SAMSN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 48 kDa band observed in Jurkat T cell lysate (calculated MW of 41.7 kDa band according to NP_071419.2). The observed size is consistent with observations with competing products.
• Control:

  SAE2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SAMSN1 Antibody

• HACS1gene with homology to KIAA0790 protein10NASH1
• Hematopoietic adaptor containing SH3 and SAM domains 1
• SAM and SH3 domain containing 1
• SAM and SH3 domain containing 2
• SAM domain, SH3 domain and nuclear localisation signals, 1
• SAM domain, SH3 domain and nuclear localization signals 1
• SAM domain, SH3 domain and nuclear localization signals protein 1
• SAM domain-containing protein SAMSN-1
• SH3D6B
• SH3-SAM adaptor protein

Background

SAMSN1 is a member of a novel gene family of putative adaptors and scaffold proteins containing SH3 and SAM (sterile alpha motif) domains. It also contains several predicted consensus nuclear localization signals and a tyrosine kinase phosphorylation motif. Northern blot analysis has revealed expression of a 2.2kb SAMSN1 transcript in human immune tissues and hematopoietic cell types including normal bone marrow, acute myeloid leukemia, and multiple myeloma; low-level expression was seen in other tissues such as heart, brain, placenta, and lung. SAMSN1 has been shown to be up-regulated by B cell activation signals and is a participant in B cell activation and differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SAMSN1 Antibody (NB300-976)(2)

Publications using NB300-976

• Kovacevic Z, Chikhani S, Lui YL et al. THE IRON-REGULATED METASTASIS SUPPRESSOR N-MYC DOWNSTREAM REGULATED GENE 1 (NDRG1) TARGETS NEDD4L, PTEN AND SMAD4 AND INHIBITS THE PI3K AND RAS SIGNALING PATHWAYS. Antioxid Redox Signal 2012-04-01 [PMID: 22462691] (WB, Human)
• Zhu YX, Benn S, Li ZH et al. The SH3-SAM Adaptor HACS1 is Up-regulated in B Cell Activation Signaling Cascades. J Exp Med 2004-09-20 [PMID: 15381729]

Bioinformatics

• Gene Symbol: SAMSN1
• Entrez:
  • 64092(Human)
• Uniprot:
  • NP_071419.2(Human)