SAMHD1 Antibody (JU56-04)

Western Blot: SAMHD1 Antibody (JU56-04) [NBP2-75655] - Analysis of SAMHD1 on THP-1 cell lysate using anti-SAMHD1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: SAMHD1 Antibody (JU56-04) [NBP2-75655] - Staining SAMHD1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SAMHD1 Antibody (JU56-04) [NBP2-75655] - Analysis of paraffin-embedded human spleen tissue using anti-SAMHD1 antibody. Counter stained with hematoxylin.

Flow Cytometry: SAMHD1 Antibody (JU56-04) [NBP2-75655] - Analysis of K562 cells with SAMHD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunocytochemistry/Immunofluorescence: SAMHD1 Antibody (JU56-04) [NBP2-75655] - Staining SAMHD1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SAMHD1 Antibody (JU56-04) [NBP2-75655] - Staining SAMHD1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SAMHD1 Antibody (JU56-04) [NBP2-75655] - Analysis of paraffin-embedded human tonsil tissue using anti-SAMHD1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SAMHD1 Antibody (JU56-04) [NBP2-75655] - Analysis of paraffin-embedded human colon cancer tissue using anti-SAMHD1 antibody. Counter stained with hematoxylin.

Product Discontinued

SAMHD1 Antibody (JU56-04) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SAMHD1 aa 577-626 / 626. (SwissProt: Q9Y3Z3 Human)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SAMHD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SAMHD1 Antibody (JU56-04)

• AGS5
• CHBL2
• DCIP
• DCIPMg11
• Dendritic cell-derived IFNG-induced protein
• EC 3.1.4.-
• HDDC1
• Monocyte protein 5
• MOP5
• MOP-5
• MOP-5AGS5
• SAM domain and HD domain 1
• SAM domain and HD domain-containing protein 1
• SAMHD1
• SBBI88

Background

Putative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SAMHD1