SAMD4B Antibody (PCRP-SAMD4B-1H3)

Immunocytochemistry/Immunofluorescence: SAMD4B Antibody (PCRP-SAMD4B-1H3) [NBP3-13749] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SAMD4B Antibody (PCRP-SAMD4B-1H3) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow Cytometry: SAMD4B Antibody (PCRP-SAMD4B-1H3) [NBP3-13749] - Flow cytometric analysis of PFA-fixed HeLa cells. SAMD4B antibody (PCRP-SAMD4B-1H3) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Protein Array: SAMD4B Antibody (PCRP-SAMD4B-1H3) [NBP3-13749] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SAMD4B Antibody (PCRP-SAMD4B-1H3).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IP, MA
• Clone: PCRP-SAMD4B-1H3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SAMD4B Antibody (PCRP-SAMD4B-1H3) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14152)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length humanSAMD4Bprotein (Uniprot: Q5PRF9)
• Localization: Nucleus
• Isotype: IgG2c
• Clonality: Monoclonal
• Host: Mouse
• Gene: SAMD4B
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: For coating, order antibody without BSA
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 75.48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react in Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for SAMD4B Antibody (PCRP-SAMD4B-1H3)

• FLJ10211
• hSmaug2
• MGC99832
• protein Smaug homolog 2
• SAM domain-containing protein 4B
• smaug 2
• smaug homolog B
• SMAUG2
• SMGB
• sterile alpha motif domain containing 4B
• sterile alpha motif domain-containing protein 4B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SAMD4B
• Uniprot:
  • Q5PRF9(Human)