S1P5/EDG-8 Antibody (282503) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with human S1P5/EDG-8 and eGFP were stained with either (A) Mouse Anti-Human S1P5/EDG-8 Monoclonal Antibody (Catalog # MAB3964) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 282503
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

S1P5/EDG-8 Antibody (282503) [Unconjugated] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human S1P₅/EDG‑8
  Met1-Asp398
  Accession # Q9H228
• Specificity: Detects human S1P₅/EDG‑8. Stains human S1P₅/EDG‑8 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: S1PR5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S1P5/EDG-8 Antibody (282503) [Unconjugated]

• EDG8
• Edg-8
• EDG8S1P receptor Edg-8
• Endothelial differentiation G-protein-coupled receptor 8
• endothelial differentiation, sphingolipid G-protein-coupled receptor, 8
• LPB4
• S1P receptor 5
• S1P5
• S1PR5
• sphingosine 1-phosphate receptor 5
• sphingosine 1-phosphate receptor EDG8
• Sphingosine 1-phosphate receptor Edg-8
• sphingosine-1-phosphate receptor 5
• SPPR-1
• SPPR-2

Background

S1P₅ is also known as EDG-8 and nerve growth factor-related G-protein-coupled receptor-1 (NRG-1). S1P₅ is a 398 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P₅ show 96% and 97% aa identity with mouse and rat S1P₅, respectively. Isoform 1 is expressed at a low level in peripheral tissues. Isoform 2 has an alternate C-terminal that is 88 aa shorter and is expressed mainly in brain, spleen, and PBMC. S1P₅ is upregulated in large granular lymphocytic leukemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: S1PR5
• Uniprot:
  • Q9H228()