S1P2/EDG-5/S1PR2 Antibody (368510) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) human S1P2/EDG-5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human S1P2/EDG-5 Monoclonal Antibody (Catalog # MAB3649) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

S1P2/EDG-5/S1PR2 Antibody (368510) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human S1P₂/EDG-5
  Met1-Val353
  Accession # AAC98919
• Specificity: Detects human S1P₂/EDG-5. Stains human S1P₂/EDG-5 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: S1PR2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S1P2/EDG-5/S1PR2 Antibody (368510) [Unconjugated]

• AGR16
• EDG5
• EDG-5
• endothelial differentiation G-protein coupled receptor 5
• endothelial differentiation, sphingolipid G-protein-coupled receptor, 5
• GPCR13
• GPCR18
• H218
• LPB2
• S1P receptor 2
• S1P receptor EDG5
• S1P receptor Edg-5
• S1P2
• S1PR2
• sphingosine 1-phosphate receptor 2
• sphingosine 1-phosphate receptor Edg-5
• sphingosine-1-phosphate receptor 2

Background

S1P₂ (sphingosine 1-phosphate receptor-2), also known as EDG-5 (endothelial differentiation, G-protein coupled receptor-5), is a 353 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P₂ show 82.5% aa identity with mouse S1P₂ and 92% aa identity with human EDG-3, the most closely related family member. Both are expressed predominantly in the lung, heart, kidney, liver, spleen, thymus, testis and brain, mediating both mitogenic and anti-apoptotic effects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S1P2/EDG-5/S1PR2 Antibody (MAB3649)(1)

Publication using MAB3649

• Capitani , Nagaja, Patrussi , Laura, Trentin , Livio, Lucherini , Orso Mar, Cannizzaro , Enrica, Migliaccio , Enrica, Frezzato , Federica, Gattazzo , Cristina, Forconi , Francesc, Pelicci , Piergius, Semenzato , Gianpiet, Baldari , Cosima T S1P1 expression is controlled by the pro-oxidant activity of p66Shc and is impaired in B-CLL patients with unfavorable prognosis. Blood, 2012-10-01;120(22):4391-9. 2012-10-01 [PMID: 23033271] (Flow Cytometry, Human)

Review for S1P2/EDG-5/S1PR2 Antibody (MAB3649) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Canine.

reviewed by: Verified Customer Flow Canine 10/12/2018

Summary

• Application: Flow Cytometry
• Sample Tested: Whole blood cells
• Species: Canine
• Lot: xw1021809A

Bioinformatics

• Gene Symbol: S1PR2
• Uniprot:
  • AAC98919()