LPAR1/LPA1/EDG-2 Antibody

Images

 
Western Blot: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - Detection of EDG2 in A549 whole cell extracts using NBP1-03363. The band at ~41 kDa position represents the target protein EDG2, whereas, the band at ~55kDa may ...read more
Immunocytochemistry/ Immunofluorescence: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
Immunohistochemistry-Paraffin: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human prostate cancer using LPAR1 antibody at 1:100 on a Bond Rx autostainer (Leica ...read more
Simple Western: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - Simple Western lane view shows a specific band for EDG2 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Mu, Bv, ShSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

LPAR1/LPA1/EDG-2 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human EDG2 (within residues 200-300). [Swiss-Prot# Q92633]
Localization
Cell membrane; Multi-pass membrane protein.
Predicted Species
Mouse (100%), Bovine (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LPAR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2.0 ug/ml
  • Simple Western 1:200
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100 -1:500
Application Notes
This EDG2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen and Western Blot, where a band is seen approx. 43 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
LPAR1/LPA1/EDG-2 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NBP1-03363 in the following applications:

Publications
Read Publications using
NBP1-03363 in the following applications:

  • IHC
    1 publication
  • WB
    2 publications

Reactivity Notes

Predicted to react with sheep, mouse, and bovine based on 100% sequence homology. Positive staining in IHC-Fr/IF with 16 um cryostat sections of embryonic day 6 (E6) chicken brain was reported by a customer. Mouse reactivity reported in scientific literature (PMID: 24147068).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LPAR1/LPA1/EDG-2 Antibody

  • EDG2
  • edg-2
  • EDG2Lysophosphatidic acid receptor Edg-2
  • endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor2,
  • GPCR26
  • GPR26
  • LPA receptor 1
  • LPA1
  • LPA-1
  • LPA1vzg-1
  • LPAR1
  • lysophosphatidic acid receptor 1
  • Mrec1.3
  • rec.1.3
  • ventricular zone gene 1
  • VZG1
  • vzg-1

Background

Lysophosphatidic acid (LPA) is a natural small-molecule lipid of glycerophosphate originating from cellular membranes and it mainly acts through binding with its specific GPC-Receptors LPA1-5 (e.g. LPA receptor EDG-2 or LPA1/EDG2, EDG4 and EDG7) which can couple to Gi, Gq, G12, and possibly Gs for regulating specific downstream signal transduction pathways including those involving activation of purinergic receptors LPA6/P2Y5, GPR87 and P2Y10, TRPV1 and PPAR gamma. EDG2 interacts with RALA, ADRBK1 etc., and in the absence of LPA, it can be found predominantly at the cell surface whereas in the presence of LPA, it co-localizes with RALA in the endocytic vesicles. It is well established that platelets, certain inflammatory cells, nerve cells, endothelial cells, cancerous cells etc. are triggered to release LPA in autocrine/paracrine fashion with the induction of diverse biologic effects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LPAR1/LPA1/EDG-2 Antibody (NBP1-03363)(3)

Review for LPAR1/LPA1/EDG-2 Antibody (NBP1-03363) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-03363:
Filter by Applications
IF
(1)
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Filter by Species
Other
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence LPAR1/LPA1/EDG-2 NBP1-03363
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reviewed by:
Eric Birgbauere
IF Other 10/20/2011
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Summary

ApplicationImmunofluorescence
Sample Tested16 um cryostat sections of embryonic day 6 (E6) chicken brain
SpeciesOther

Blocking

Blocking DetailsBlocking Buffer: PBS + 10% goat serum + 1% BSA + 0.05% NaN3

Primary Anitbody

Dilution RatioIncubation Dilution Buffer: Diluted in PBS + 1% goat serum + 1% BSA, Dilution Ratio: 1:250, Incubation Time: overnight, Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Invitrogen Molecular Probes AlexaFluor 568 Goat anti-rabbit, Secondary Ab Dilution: 1:400

Details

Detection NotesDetection Method: fluorescence, Pos Ctrl: stained w/anti-neurofilament ab, Neg Ctrl: sections w/o primary ab
Fixation DetailsSample Preparation: 4% PFA in PBS pH 7.4

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LPAR1/LPA1/EDG-2 Antibody (NBP1-03363) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-03363AF350
Alexa Fluor 405 NBP1-03363AF405
Alexa Fluor 488 NBP1-03363AF488
Alexa Fluor 532 NBP1-03363AF532
Alexa Fluor 594 NBP1-03363AF594
Alexa Fluor 647 NBP1-03363AF647
Alexa Fluor 700 NBP1-03363AF700
Alexa Fluor 750 NBP1-03363AF750
Biotin NBP1-03363B
DyLight 350 NBP1-03363UV
DyLight 405 NBP1-03363V
DyLight 488 NBP1-03363G
DyLight 550 NBP1-03363R
DyLight 594 NBP1-03363DL594
DyLight 650 NBP1-03363C
DyLight 680 NBP1-03363FR
DyLight 755 NBP1-03363IR
FITC NBP1-03363F
HRP NBP1-03363H
Janelia Fluor 549 NBP1-03363JF549
Janelia Fluor 646 NBP1-03363JF646

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Recent Reviews

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5
0
4
1
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2
0
1
0

Eric Birgbauere
10/20/2011
Application: IF
Species: Other

Bioinformatics

Gene Symbol LPAR1
Entrez
Uniprot