Western Blot: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - Detection of EDG2 in A549 whole cell extracts using NBP1-03363. The band at ~41 kDa position represents the target protein EDG2, whereas, the band at ~55kDa may ...read more
Immunocytochemistry/ Immunofluorescence: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
Immunohistochemistry-Paraffin: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human prostate cancer using LPAR1 antibody at 1:100 on a Bond Rx autostainer (Leica ...read more
Simple Western: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - Simple Western lane view shows a specific band for EDG2 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
This EDG2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen and Western Blot, where a band is seen approx. 43 kDa.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
43 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Predicted to react with sheep, mouse, and bovine based on 100% sequence homology. Positive staining in IHC-Fr/IF with 16 um cryostat sections of embryonic day 6 (E6) chicken brain was reported by a customer. Mouse reactivity reported in scientific literature (PMID: 24147068).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Lysophosphatidic acid (LPA) is a natural small-molecule lipid of glycerophosphate originating from cellular membranes and it mainly acts through binding with its specific GPC-Receptors LPA1-5 (e.g. LPA receptor EDG-2 or LPA1/EDG2, EDG4 and EDG7) which can couple to Gi, Gq, G12, and possibly Gs for regulating specific downstream signal transduction pathways including those involving activation of purinergic receptors LPA6/P2Y5, GPR87 and P2Y10, TRPV1 and PPAR gamma. EDG2 interacts with RALA, ADRBK1 etc., and in the absence of LPA, it can be found predominantly at the cell surface whereas in the presence of LPA, it co-localizes with RALA in the endocytic vesicles. It is well established that platelets, certain inflammatory cells, nerve cells, endothelial cells, cancerous cells etc. are triggered to release LPA in autocrine/paracrine fashion with the induction of diverse biologic effects.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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