This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source
Wheat germ
Protein/Peptide Type
Recombinant Protein
Gene
S100A9
Purity
>80% by SDS-PAGE and Coomassie blue staining
Applications/Dilutions
Dilutions
ELISA
Immunoaffinity Purification
Protein Array
Western Blot
Theoretical MW
38.28 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0.
Purity
>80% by SDS-PAGE and Coomassie blue staining
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Recombinant Human S100A9 Protein
CAGBMigration inhibitory factor-related protein 14
Calgranulin B
calgranulin-B
Calprotectin L1H subunit
CFAGMRP-14,60B8AG
CGLB
L1AG
LIAG
MAC387
MIF
MRP-14
MRP14Leukocyte L1 complex heavy chain
NIF
P14
protein S100-A9
S100 calcium binding protein A9 (calgranulin B)
S100 calcium binding protein A9
S100 calcium-binding protein A9 (calgranulin B)
S100 calcium-binding protein A9
S100A9
Background
S100A9( AAH47681, 1 a.a. - 115 a.a.) recombinant protein with GST.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for S100A9 Recombinant Protein (H00006280-P01). (Showing 1 - 2 of 2 FAQs).
Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?
Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a> information can be found using this link.
We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.
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