S100A9 Antibody [142Nd]

Western Blot: S100A9 Antibody [NBP1-18843] - Staining of Human Bone Marrow (A) with antibody at 1 ug/mL and Gastrointestinal cancer (B) lysate with antibody at 0.5 ug/mL and negative control HepG2 (C) lysate. 5 ug protein in RIPA buffer. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: S100A9 Antibody [NBP1-18843] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunocytochemistry/Immunofluorescence: S100A9 Antibody [NBP1-18843] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunocytochemistry/Immunofluorescence: Goat Polyclonal S100A9 Antibody [NBP1-18843] - Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Flow Cytometry: S100A9 Antibody [NBP1-18843] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Paraffin: Goat Polyclonal S100A9 Antibody [NBP1-18843] - (7µg/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Control: Goat Polyclonal S100A9 Antibody [NBP1-18843] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

Immunohistochemistry-Paraffin: S100A9 Antibody [NBP1-18843] - Staining of paraffin embedded human lung. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: 142Nd
• Concentration: 0.5 mg/ml

S100A9 Antibody [142Nd] Summary

• Immunogen: This S100A9 antibody was developed against a peptide with sequence C-DTNADKQLSFEEF corresponding to internal region according to NP_002956.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: S100A9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Detection limit 1:8000
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 0.5 - 2 ug/mL
• Application Notes: Approx 13-14kDa band observed in Human Bone Marrow lysates and approx 14-15kDa in Human Gastrointestinal cancer lysates (calculated MW of 13.2kDa according to NP_002956.1).
  
  IHC: Paraffin embedded Human Lung. Recommended concentration: 2-4 ug/ml. Paraffin embedded Human Spleen. Recommended concentration: 6-7 ug/ml.
• Theoretical MW: 13 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for S100A9 Antibody [142Nd]

• CAGBMigration inhibitory factor-related protein 14
• Calgranulin B
• calgranulin-B
• Calprotectin L1H subunit
• CFAGMRP-14,60B8AG
• CGLB
• L1AG
• LIAG
• MAC387
• MIF
• MRP-14
• MRP14Leukocyte L1 complex heavy chain
• NIF
• P14
• protein S100-A9
• S100 calcium binding protein A9 (calgranulin B)
• S100 calcium binding protein A9
• S100 calcium-binding protein A9 (calgranulin B)
• S100 calcium-binding protein A9
• S100A9

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S100A9 Antibody (NBP1-18843)(2)

Publications using NBP1-18843

• Corbin BD, Seeley EH, Raab A et al. Metal chelation and inhibition of bacterial growth in tissue abscesses. Science 2008-02-15 [PMID: 18276893]
• McCormick MM, Rahimi F, Bobryshev YV et al. S100A8 and S100A9 in human arterial wall. Implications for atherogenesis. J Biol Chem 2005-12-01 [PMID: 16216873]

FAQs for S100A9 Antibody (NBP1-18843). (Showing 1 - 2 of 2 FAQs).

1. Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?
   • Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a> information can be found using this link.
2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
   • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

Bioinformatics

• Gene Symbol: S100A9
• Entrez:
  • 6280(Human)
• Uniprot:
  • NP_002956.1(Human)