S100A9 Antibody [Alexa Fluor® 700]

• Applications: WB, IHC, Flow, ICC/IF, KO
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

S100A9 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human S100A9
  The2-Pro114
  Accession # P06702
• Specificity: Detects human S100A9 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse S100A9 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Human S100A9 (also MRP-14, Calgranulin-B, and p14) is a 14 kDa member of the S100 family of EF-hand calcium-binding proteins.  It is 114 amino acids (aa) in length and contains short sequential modules.  There is an N-terminal helical region, followed by a calcium-binding EF-hand domain, two more helical regions, a second EF-hand domain, and three additional helical regions.  S100A9 will noncovalently heterodimerize with S100A8.  In the presence of calcium, this heterodimer will form a heterotetramer. S100A9 is expressed in granulocytes, monocytes, and macrophages during acute and chronic inflammation.  Human S100A9 shares 62% and 57% aa identity with rat and mouse S100A9, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for S100A9 Antibody (AF5578N). (Showing 1 - 2 of 2 FAQ).

1. Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?
   • Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a> information can be found using this link.
2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
   • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.