S100A9 Antibody Pair

Sandwich ELISA: S100A9 Antibody Pair [H00006280-AP11] - Detection sensitivity ranging from 0.03 ng/ml to 100 ng/ml.

• Reactivity: Hu
• Applications: ELISA
• Standard Curve Range: 0.03 ng/ml to 100 ng/ml

S100A9 Antibody Pair Summary

• Description: Quality control test: Standard curve using recombinant protein (H00006280-P01) as an analyte. This antibody pair set comes with matched antibody pair to detect and quantify protein level of human S100A9.
• Standard Curve Range: 0.03 ng/ml to 100 ng/ml
• Assay Type: Sandwich ELISA
• Gene: S100A9

Applications/Dilutions

• Dilutions:
  • Sandwich ELISA 0.03 ng/ml - 100 ng/ml
• Application Notes: Reagents are sufficient for at least 1-2 x 96 well plates using recommended protocols.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 1x PBS, pH 7.4

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for S100A9 Antibody Pair

• CAGBMigration inhibitory factor-related protein 14
• Calgranulin B
• calgranulin-B
• Calprotectin L1H subunit
• CFAGMRP-14,60B8AG
• CGLB
• Human S100A9 Capture Antibody
• L1AG
• LIAG
• MAC387
• MIF
• MRP-14
• MRP14Leukocyte L1 complex heavy chain
• NIF
• P14
• protein S100-A9
• S100 calcium binding protein A9 (calgranulin B)
• S100 calcium binding protein A9
• S100 calcium-binding protein A9 (calgranulin B)
• S100 calcium-binding protein A9
• S100A9

Background

This antibody pair set comes with matched antibody pair to detect and quantify protein level of human S100A9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Publications for S100A9 Antibody Pair (H00006280-AP11)(1)

Publication using H00006280-AP11

• Sun W, Xing B, Guo L et al. Quantitative Proteomics Analysis of Tissue Interstitial Fluid for Identification of Novel Serum Candidate Diagnostic Marker for Hepatocellular Carcinoma. Sci Rep 2016-05-24 [PMID: 27216119] (WB)

FAQs for S100A9 Antibody Pair (H00006280-AP11). (Showing 1 - 2 of 2 FAQ).

1. Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?
   • Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a> information can be found using this link.
2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
   • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

Bioinformatics

• Gene Symbol: S100A9