S100A16 Antibody

S100A16 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 10 µg/mL Sheep Anti-Human S100A16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5180) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human S100A16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5180) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A16 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

S100A16 Antibody Summary

• Immunogen: E. coli-derived recombinant human S100A16
  Ser2-Ser103
  Accession # Q96FQ6
• Specificity: Detects human S100A16 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 20% cross-reactivity with recombinant mouse S100A16 and less than 1% cross-reactivity with recombinant human (rh) S100A13 and rhS100B.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: S100A16
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100A16 Antibody

• AAG13
• Aging-associated gene 13 protein
• aging-associated protein 13
• DT1P1A7
• MGC17528
• protein S100-A16
• Protein S100-F
• S100 calcium binding protein A16
• S100A16
• S100F
• S100FS100 calcium-binding protein A16

Background

S100A16 (also S100F) is a 12 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. By Northern blot, S100A16 is found in multiple tissue types; in brain, it is specifically found in astrocytes. Intracellularly, S100A16 is both nuclear and cytoplasmic and apparently exists as a homodimer, trimer and tetramer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: S100A16
• Uniprot:
  • Q96FQ6()