Ryanodine Receptor 1 Antibody

Western Blot: Ryanodine Receptor 1 Antibody [46140002] - Human breast cancer cell MDA-MB-231 was treated with carboplatin for 72 hours and the expression of RyR1 was detected by Western blot. Image from verified customer review.

ELISA: Ryanodine Receptor 1 Antibody [46140002]

Product Discontinued

Ryanodine Receptor 1 Antibody Summary

• Immunogen: This antibody is specific for the C Terminus Region of the target protein.
• Specificity: This product is specific for Human RYR1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RYR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunoprecipitation
  • Western Blot
• Application Notes: Use in immunoprecipitation reported in scientific literature (PMID: 28228260).

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Potassium Phosphate (pH 7.0), 0.15 M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for Ryanodine Receptor 1 Antibody

• CCO
• central core disease of muscle
• MHS
• MHS1
• PPP1R137
• protein phosphatase 1, regulatory subunit 137
• ryanodine receptor 1 (skeletal)
• Ryanodine Receptor 1
• ryanodine receptor type1
• RYDR
• RYR
• RyR1
• RYR-1
• sarcoplasmic reticulum calcium release channel
• Skeletal muscle calcium release channel
• skeletal muscle ryanodine receptor
• Skeletal muscle-type ryanodine receptor
• SKRR
• type 1-like ryanodine receptor

Background

The Ryanodine Receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR 1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR 2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ryanodine Receptor 1 Antibody (46140002)(3)

Publications using 46140002

• Lu H, Chen I, Shimoda LA et al. Chemotherapy-Induced Ca 2+ Release Stimulates Breast Cancer Stem Cell Enrichment Cell Rep. 2017-02-21 [PMID: 28228260] (WB, IP, Human)
  
  Details:
  The positive impact of HIF inhibitors on breast cancer chemotherapy is explored through GSTO1 knockdown.
• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Review for Ryanodine Receptor 1 Antibody (46140002) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Haiquan Lu WB Human 09/21/2018

Summary

• Application: Western Blot
• Sample Tested: Breast cancer cells
• Species: Human
• Lot: T01637A01

Bioinformatics

• Gene Symbol: RYR1
• Entrez:
  • 6261(Human)
• Uniprot:
  • P21817(Human)