RSK3 Antibody (1F6) - Azide and BSA Free

Western Blot: RSK3 Antibody (1F6) [H00006196-M01] - Analysis of RPS6KA2 expression in transfected 293T cell line by RPS6KA2 monoclonal antibody (M01), clone 1F6.Lane 1: RPS6KA2 transfected lysate(83.2 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: RSK3 Antibody (1F6) [H00006196-M01] - Analysis of monoclonal antibody to RPS6KA2 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: RSK3 Antibody (1F6) [H00006196-M01] - Analysis of monoclonal antibody to RPS6KA2 on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 3 ug/ml.

Western Blot: RSK3 Antibody (1F6) [H00006196-M01] - Analysis of RPS6KA2 over-expressed 293 cell line, cotransfected with RPS6KA2 Validated Chimera RNAi ( Cat # H00006196-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RPS6KA2 monoclonal antibody (M01) clone 1F6 (Cat # H00006196-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: RSK3 Antibody (1F6) [H00006196-M01] - RPS6KA2 monoclonal antibody (M01), clone 1F6 Analysis of RPS6KA2 expression in Hela S3 NE.

Proximity Ligation Assay: RSK3 Antibody (1F6) [H00006196-M01] - Analysis of protein-protein interactions between MAPK3 and RPS6KA2. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-RPS6KA2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Func, ICC/IF, IHC, KD
• Clone: 1F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RSK3 Antibody (1F6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RPS6KA2 (AAH02363, 631 a.a. ~ 733 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YALSGGNWDSISDAAKDVVSKMLHVDPHQRLTAMQVLKHPWVVNREYLSPNQLSRQDVHLVKGAMAATYFALNRTPQAPRLEPVLSSNLAQRRGMKRLTSTRL
• Specificity: RPS6KA2 (1F6)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RPS6KA2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Proximity Ligation Assay
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recominant protein and cell lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.
• Control:

  HeLa Nuclear Cell Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23913705). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RSK3 Antibody (1F6) - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.1
• HU-2
• MAP kinase-activated protein kinase 1c
• MAPK-activated protein kinase 1c
• MAPKAP kinase 1c
• MAPKAPK1C
• MAPKAPK-1c
• p90-RSK 2
• p90RSK2
• p90-RSK3
• ribosomal protein S6 kinase alpha 2
• ribosomal protein S6 kinase alpha-2
• ribosomal protein S6 kinase, 90kD, polypeptide 2
• ribosomal protein S6 kinase, 90kDa, polypeptide 2
• Ribosomal S6 kinase 3,90 kDa ribosomal protein S6 kinase 2
• RPS6KA2
• RSK
• RSK3
• RSK-3
• RSK3pp90RSK3
• S6K-alpha
• S6K-alpha2
• S6K-alpha-2

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RSK3 Antibody (H00006196-M01)(1)

Publication using H00006196-M01

• Passariello CL, Gayanilo M, Kritzer MD et al. p90 ribosomal S6 kinase 3 contributes to cardiac insufficiency in alpha-tropomyosin Glu180Gly transgenic mice. Am J Physiol Heart Circ Physiol. 2013-10-01 [PMID: 23913705] (Mouse)

Bioinformatics

• Gene Symbol: RPS6KA2
• Entrez:
  • 6196(Human)
  • 20112(Mouse)
  • 117269(Rat)
• OMIM:
  • 601685(Human)
• Uniprot:
  • AAH02363(Human)