RSK1 [p Thr359, p Ser363] Antibody (SU03-65)

Western Blot: RSK1 [p Thr359, p Ser363] Antibody (SU03-65) [NBP2-67480] - Western blot analysis of RSK1 on different lysates using anti-RSK1 antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: 293 Lane 3: MCF-7

Immunocytochemistry/Immunofluorescence: RSK1 [p Thr359, p Ser363] Antibody (SU03-65) [NBP2-67480] - Staining Phospho-RSK1 p90(T359+S363) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RSK1 [p Thr359, p Ser363] Antibody (SU03-65) [NBP2-67480] - Analysis of paraffin-embedded mouse liver tissue using anti-Phospho-RSK1 p90(T359+S363) antibody. Counter stained with hematoxylin.

Western Blot: RSK1 [p Thr359, p Ser363] Antibody (SU03-65) [NBP2-67480] - Analysis of Phospho-RSK1 p90(T359+S363) on different lysates using anti-Phospho-RSK1 p90(T359+S363) antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: 293 Lane 3: MCF-7

Immunocytochemistry/Immunofluorescence: RSK1 [p Thr359, p Ser363] Antibody (SU03-65) [NBP2-67480] - Staining Phospho-RSK1 p90(T359+S363) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: RSK1 [p Thr359, p Ser363] Antibody (SU03-65) [NBP2-67480] - Staining Phospho-RSK1 p90(T359+S363) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RSK1 [p Thr359, p Ser363] Antibody (SU03-65) [NBP2-67480] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-RSK1 p90(T359+S363) antibody. Counter stained with hematoxylin.

Product Discontinued

RSK1 [p Thr359, p Ser363] Antibody (SU03-65) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding RSK1 aa 341-390 / 735. (SwissProt: Q15418 Human; SwissProt: P18653 Mouse; SwissProt: Q63531 Rat)
• Modification: p Thr359, p Ser363
• Localization: Nucleus, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RPS6KA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RSK1 [p Thr359, p Ser363] Antibody (SU03-65)

• 90kD, polypeptide 1)
• EC 2.7.11
• EC 2.7.11.1,90 kDa ribosomal protein S6 kinase 1
• HU-1
• MAP kinase-activated protein kinase 1a
• MAPK-activated protein kinase 1a
• MAPKAP kinase 1a
• MAPKAPK-1a
• MAPKAPL1A
• p90RSK1
• p90S6K
• ribosomal protein S6 kinase alpha 1
• ribosomal protein S6 kinase alpha-1
• ribosomal protein S6 kinase, 90kD, polypeptide 1
• ribosomal protein S6 kinase, 90kDa, polypeptide 1
• Ribosomal S6 kinase 1
• RPS6KA1
• RSK
• RSK1
• RSK-1
• RSK1p90-RSK 1
• S6K-alpha 1
• S6K-alpha-1

Background

Rsk-1 (p90-Rsk-1) is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk-1, Rsk-2 and Rsk-3. These are broadly expressed serine/threonine protein kinases (1) activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2 (2). p90-Rsk-1 is activated by MAPK in vitro and in vivo via phosphorylation (3). Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB (2,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KA1