RSK1 Antibody (JE48-76)

Images

 
Western Blot: RSK1 Antibody (JE48-76) [NBP2-80423] - Analysis of RSK1 on Daudi cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary ...read more
Immunocytochemistry/ Immunofluorescence: RSK1 Antibody (JE48-76) [NBP2-80423] - Staining of RSK1 p90 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room ...read more
Immunohistochemistry-Paraffin: RSK1 Antibody (JE48-76) [NBP2-80423] - Analysis in mouse colon tissue using RSK1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH ...read more
Flow Cytometry: RSK1 Antibody (JE48-76) [NBP2-80423] - Analysis of RSK1 p90 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary ...read more
Immunohistochemistry-Paraffin: RSK1 Antibody (JE48-76) [NBP2-80423] - Analysis in rat testis tissue using RSK1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH ...read more
Immunohistochemistry-Paraffin: RSK1 Antibody (JE48-76) [NBP2-80423] - Analysis in human appendix tissue using RSK1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer ...read more
Immunohistochemistry-Paraffin: RSK1 Antibody (JE48-76) [NBP2-80423] - Analysis in human prostate cancer tissue using RSK1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate ...read more
Immunohistochemistry-Paraffin: RSK1 Antibody (JE48-76) [NBP2-80423] - Analysis in human small intestine tissue using RSK1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
JE48-76
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

RSK1 Antibody (JE48-76) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant protein within N-terminal human RSK1 p90.
Localization
Cytoplasm. Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
RPS6KA1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
1X TBS(pH7.4), 1%BSA, 50%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for RSK1 Antibody (JE48-76)

  • 90kD, polypeptide 1)
  • EC 2.7.11
  • EC 2.7.11.1,90 kDa ribosomal protein S6 kinase 1
  • HU-1
  • MAP kinase-activated protein kinase 1a
  • MAPK-activated protein kinase 1a
  • MAPKAP kinase 1a
  • MAPKAPK-1a
  • MAPKAPL1A
  • p90RSK1
  • p90S6K
  • ribosomal protein S6 kinase alpha 1
  • ribosomal protein S6 kinase alpha-1
  • ribosomal protein S6 kinase, 90kD, polypeptide 1
  • ribosomal protein S6 kinase, 90kDa, polypeptide 1
  • Ribosomal S6 kinase 1
  • RPS6KA1
  • RSK
  • RSK1
  • RSK-1
  • RSK1p90-RSK 1
  • S6K-alpha 1
  • S6K-alpha-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RPS6KA1