RSK Antibody - Pan Specific

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, TS1 mouse helper T cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat RSK Pan Specific, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2056) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for RSK Pan Specific, at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

RSK Antibody - Pan Specific Summary

• Immunogen: Human RSK2 synthetic peptide
• Specificity: Detects endogenous human, mouse and rat RSK family isoforms in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RSK Antibody - Pan Specific

• HU-1;MAPKAPK1;MAPKAPK1A;p90Rsk;Ribosomal protein S6 kinase alpha-1;RPS6KA1;RSK;RSK1
• RSK

Background

This peptide sequence is identical to amino acids 162‑184 of human RSK3 (RPS6KA2). The sequence differs at one position from amino acids 165‑187 of human RSK1 (RPS6KA1) and at two positions from amino acids 176‑198 of human RSK4 (RPS6KA6). The immunogen is also highly homologous to the corresponding sequences of mouse and rat RSK family isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RSK Antibody (AF2056)(2)

Publications using AF2056

• A Bishnu, P Phadte, A Dhadve, A Sakpal, B Rekhi, P Ray Molecular imaging of the kinetics of hyperactivated ERK1/2-mediated autophagy during acquirement of chemoresistance Cell Death & Disease, 2021-02-08;12(2):161. 2021-02-08 [PMID: 33558461] (Western Blot, Human)
• Mathew S, Nieves B, Sequeira S, Sambandamoorthy S, Pumiglia K, Larsen M, Laflamme S Integrins promote cytokinesis through the RSK signaling axis. J Cell Sci, 2013-11-27;127(0):534-45. 2013-11-27 [PMID: 24284076] (Western Blot, Human)