RRAS Antibody (2E12) - Azide and BSA Free

Western Blot: RRAS Antibody (2E12) [H00006237-M01] - RRAS monoclonal antibody (M01), clone 2E12 Analysis of RRAS expression in HeLa.

Immunoprecipitation: RRAS Antibody (2E12) [H00006237-M01] - Analysis of RRAS transfected lysate using anti-RRAS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RRAS MaxPab rabbit polyclonal antibody.

ELISA: RRAS Antibody (2E12) [H00006237-M01] - RRAS Antibody (2E12) [H00006237-M01] - Detection limit for recombinant GST tagged RRAS is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 2E12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RRAS Antibody (2E12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RRAS (AAH16286, 109 a.a. ~ 218 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AINDRQSFNEVGKLFTQILRVKDRDDFPVVLVGNKADLESQRQVPRSEASAFGASHHVAYFEASAKLRLNVDEAFEQLVRAVRKYQEQELPPSPPSAPRKKGGGCPCVLL
• Specificity: RRAS - related RAS viral (r-ras) oncogene homolog
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RRAS
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RRAS Antibody (2E12) - Azide and BSA Free

• Oncogene RRAS
• p23
• ras-related protein R-Ras
• related RAS viral (r-ras) oncogene homolog

Background

R-Ras is a member of the Ras superfamily of GTPases. Ras proteins function as signaling focal points that are activated by convergent signaling pathways intiated by extracellular stimuli (reviewed in Mitin et al, 2005). Activated Ras proteins in turn regulate a diversity of downstream cytoplasmic signaling cascades. Many members of the Ras superfamily are implicated in the regulation of hematopoietic cells, with roles in growth, survival, differentiation, cytokine production, chemotaxis, vesicle-trafficking, and phagocytosis. The Ras superfamily of proteins includes over 150 small GTPases (distinguished from the large, heterotrimeric GTPases, the G-proteins). It comprises six subfamilies, the Ras, Rho, Ran, Rab, Arf, and Kir/Rem/Rad subfamilies. They exhibit considerable overall amino acid identities, especially in the regions interacting with the guanine nucleotide exchange factors that catalyze their activation. Recognizes R-Ras; human R-Ras is a 218 amino acid protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RRAS Antibody (H00006237-M01)(4)

Publications using H00006237-M01

• Shannon W, Nicole B, Amanda P et al. R-Ras subfamily proteins elicit distinct physiologic effects and phosphoproteome alterations in neurofibromin-null MPNST cells. Cell Commun Signal. 2021-09-16 [PMID: 34530870]
• Jose H, Masanobu K R-Ras Deficiency in Pericytes Causes Frequent Microphthalmia and Perturbs Retinal Vascular Development. J Vasc Res. 2021-04-19 [PMID: 33873190]
• Junko S, Carole P, Linyuan C et al. High endothelial venules accelerate naive T cell recruitment by tumor necrosis factor-mediated R-Ras up-regulation. Am J Pathol. 2020-11-04 [PMID: 33159887]
• Perrot CY, Sawada J, Komatsu M. et al. Prolonged activation of cAMP signaling leads to endothelial barrier disruption via transcriptional repression of RRAS. FASEB J 2018-05-18 [PMID: 29775418]

Bioinformatics

• Gene Symbol: RRAS
• Entrez:
  • 6237(Human)
• OMIM:
  • 165090(Human)
• Uniprot:
  • AAH16286(Human)