RPS3A Antibody

Western Blot: RPS3A Antibody [NBP2-20225] - Sample (30 ug of whole cell lysate) A: A431 B: Raji 12% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: RPS3A Antibody [NBP2-20225] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: RPS3A Antibody [NBP2-20225] - Rat brain. RPS3A antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: RPS3A Antibody [NBP2-20225] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry: RPS3A Antibody [NBP2-20225] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using RPS3A antibody (NBP2-20225) at 1:300 dilution.

Western Blot: RPS3A Antibody [NBP2-20225] - Sample (30 ug of whole cell lysate) A: zebrafish eye 12% SDS PAGE NBP2-20225 diluted at 1:1000

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RPS3A Antibody Summary

• Immunogen: Full length human RPS3A Recombinant protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS3A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RPS3A Antibody

• 40S ribosomal protein S3a
• FTE1
• fte-1
• MFTL
• MGC23240
• ribosomal protein S3A
• v-fos transformation effector protein 1
• v-fos transformation effector protein

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. Transcript variants utilizing alternative transcription start sites have been described. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for RPS3A Antibody (NBP2-20225)(1)

Publication using NBP2-20225

• Arpit Dheeraj, Fernando Jose Garcia Marques, Dhanir Tailor, Abel Bermudez, Angel Resendez, Mallesh Pandrala, Benedikt Grau, Praveen Kumar, Carrsyn B. Haley, Alexander Honkala, Praveen Kujur, Stefanie S. Jeffrey, Sharon Pitteri, Sanjay V. Malhotra Inhibition of protein translational machinery in triple-negative breast cancer as a promising therapeutic strategy Cell Reports Medicine 2024-05-09 [PMID: 38729158]

Bioinformatics

• Gene Symbol: RPS3A
• Uniprot:
  • P61247(Human)