RPS13 Antibody

Western Blot: RPS13 Antibody [NBP2-93953] - Analysis of extracts of various cell lines, using RPS13 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 20s.

Immunocytochemistry/Immunofluorescence: RPS13 Antibody [NBP2-93953] - Analysis of U-2 OS cells using RPS13 at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: RPS13 Antibody [NBP2-93953] - Human placenta using RPS13 antibody at dilution of 1:100 (40x lens).

Immunocytochemistry/Immunofluorescence: RPS13 Antibody [NBP2-93953] - Analysis of L929 cells using RPS13 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RPS13 Antibody Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 79-151 of human RPS13 (NP_001008.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS13
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for RPS13 Antibody

• ribosomal protein S13,40S ribosomal protein S13

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S15P family of ribosomal proteins. It is located in the cytoplasm. The protein has been shown to bind to the 5.8S rRNA in rat. The gene product of the E. coli ortholog (ribosomal protein S15) functions at early steps in ribosome assembly. This gene is co-transcribed with two U14 small nucleolar RNA genes, which are located in its third and fifth introns. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS13