RPS11 Antibody

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Biological Strategies: Western Blot: RPS11 Antibody [NBP2-22288] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22288 used for WB at 0.1 ug/ml. ...read more
Immunohistochemistry-Paraffin: RPS11 Antibody [NBP2-22288] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- RPS11 used at a dilution of 1:1,000 (1ug/ml). Detection: ...read more
Immunoprecipitation: RPS11 Antibody [NBP2-22288] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-RPS11 antibody NBP2-22288 used for IP at 6 ug/mg lysate. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, XpSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

RPS11 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 1 and 50 of human Ribosomal Protein S11 using the numbering given in entry NP_001006.1 (GeneID 6205).
Predicted Species
Rat (100%), Canine (100%), Xenopus (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPS11
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 -1:2000
  • Immunohistochemistry-Paraffin 1:500 -1:2000
  • Immunoprecipitation 2 - 10 ug/mg
  • Western Blot 1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RPS11 Antibody

  • ribosomal protein S11,40S ribosomal protein S11

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S17P family of ribosomal proteins. It is located in the cytoplasm. The gene product of the E. coli ortholog (ribosomal protein S17) is thought to be involved in the recognition of termination codons. This gene is co-transcribed with a small nucleolar RNA gene, which is located in its third intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Array NBP2-22288

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Bioinformatics

Gene Symbol RPS11
Uniprot