RPL9 Antibody - BSA Free

Western Blot: RPL9 Antibody [NBP2-20219] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: RPL9 Antibody [NBP2-20219] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: RPL9 Antibody [NBP2-20219] - Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.

Western Blot: RPL9 Antibody [NBP2-20219] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 C: HCT116 12% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: RPL9 Antibody [NBP2-20219] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Immunohistochemistry: RPL9 Antibody [NBP2-20219] - Immunohistochemical analysis (whole mount) of zebrafish embryo, using RPL9 antibody (NBP2-20219) at 1:200 dilution.

Western Blot: RPL9 Antibody [NBP2-20219] - Sample (30 ug of whole cell lysate) A: Zebrafish muscle 12% SDS PAGE NBP2-20219 diluted at 1:1000

Immunohistochemistry-Paraffin: RPL9 Antibody [NBP2-20219] - RPL9 antibody detects RPL9 protein at retinal pigment epithelium in mouse eye by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. RPL9 antibody (NBP2-20219) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Pm, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RPL9 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RPL9 Antibody - BSA Free (NBP2-20219) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-RPL9 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human RPL9. The exact sequence is proprietary.
• Predicted Species: Rhesus Macaque (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPL9
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RPL9 Antibody - BSA Free

• DKFZp313J1510
• FLJ27456
• MGC15545
• NPC-A-16
• ribosomal protein L9,60S ribosomal protein L9
• RPL9P7
• RPL9P8
• RPL9P9

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L6P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for RPL9 Antibody (NBP2-20219)(1)

Publication using NBP2-20219

• Arpit Dheeraj, Fernando Jose Garcia Marques, Dhanir Tailor, Abel Bermudez, Angel Resendez, Mallesh Pandrala, Benedikt Grau, Praveen Kumar, Carrsyn B. Haley, Alexander Honkala, Praveen Kujur, Stefanie S. Jeffrey, Sharon Pitteri, Sanjay V. Malhotra Inhibition of protein translational machinery in triple-negative breast cancer as a promising therapeutic strategy Cell Reports Medicine 2024-05-09 [PMID: 38729158]

Bioinformatics

• Gene Symbol: RPL9
• Uniprot:
  • P32969(Human)