RPL10A Overexpression Lysate

Images

 
Western Blot: RPL10A Overexpression Lysate (Adult Normal) [NBL1-15500] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RPL10A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RPL10A Overexpression Lysate Summary

Description
Transient overexpression lysate of ribosomal protein L10a (RPL10A) The lysate was created in HEK293T cells, using Plasmid ID RC209987 and based on accession number NM_007104. The protein contains a C-MYC/DDK Tag.
Gene
RPL10A

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
24.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RPL10A Overexpression Lysate

  • CSA-19
  • Csa-19,60S ribosomal protein L10a
  • NEDD6
  • NEDD-6
  • Neural precursor cell expressed developmentally down-regulated protein 6
  • ribosomal protein L10a

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. The expression of this gene is downregulated in the thymus by cyclosporin-A (CsA), an immunosuppressive drug. Studies in mice have shown that the expression of the ribosomal protein L10a gene is downregulated in neural precursor cells during development. This gene previously was referred to as NEDD6 (neural precursor cell expressed, developmentally downregulated 6), but it has been renamed RPL10A (ribosomal protein 10a). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-84037
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-84744
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-20212
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-67471
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-2277
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NBP2-32572
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-84222
Species: Hu, Rt
Applications: IHC, IHC-P, WB
H00001958-M03
Species: Hu, Mu, Rb, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-06032
Species: Hu
Applications: PEP-ELISA, WB
NBP2-29463
Species: Pm, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB600-235
Species: Hu, Mu, Po
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-20216
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-57476
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
H00006124-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
NBP1-31413
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
202-IL
Species: Hu
Applications: BA
201-LB/CF
Species: Hu
Applications: BA
AF2780
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, WB
H00006187-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBL1-15500
Species: Hu
Applications: WB

Publications for RPL10A Lysate (NBL1-15500) (0)

There are no publications for RPL10A Lysate (NBL1-15500).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for RPL10A Lysate (NBL1-15500) (0)

There are no reviews for RPL10A Lysate (NBL1-15500). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RPL10A Lysate (NBL1-15500) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional RPL10A Products

Bioinformatics Tool for RPL10A Lysate (NBL1-15500)

Discover related pathways, diseases and genes to RPL10A Lysate (NBL1-15500). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for RPL10A Lysate (NBL1-15500)

Discover more about diseases related to RPL10A Lysate (NBL1-15500).
 

Pathways for RPL10A Lysate (NBL1-15500)

View related products by pathway.

PTMs for RPL10A Lysate (NBL1-15500)

Learn more about PTMs related to RPL10A Lysate (NBL1-15500).
 

Research Areas for RPL10A Lysate (NBL1-15500)

Find related products by research area.

Blogs on RPL10A

There are no specific blogs for RPL10A, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our RPL10A Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol RPL10A