Recombinant Human RPB9 His Protein

SDS-Page: Recombinant Human RPB9 Protein [NBP2-23346]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human RPB9 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-125 of Human RPB9
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEPDGTY EPGFVGIRFC QECNNMLYPK EDKENRILLY ACRNCDYQQE ADNSCIYVNK ITHEVDELTQ IIADVSQDPT LPRTEDHPCQ KCGHKEAVFF QSHSARAEDA MRLYYVCTAP HCGHRWTE

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: POLR2I
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human RPB9 His Protein

• DNA-directed RNA polymerase II 14.5 kDa polypeptide
• DNA-directed RNA polymerase II subunit I
• DNA-directed RNA polymerase II subunit RPB9
• hRPB14.5
• polymerase (RNA) II (DNA directed) polypeptide I (14.5kD)
• polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
• RNA polymerase II 14.5 kDa subunit
• RNA polymerase II subunit B9
• RPB14.5
• RPB9

Background

POLR2I, also known as RPB9, belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family. This protein is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Also it is synthesizes mRNA precursors and many functional non-coding RNAs. Recombinant human PORL2I protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: POLR2I