RPA2 Antibody (9H8)

Images

 
Immunohistochemistry: RPA2 Antibody (9H8) [NB600-565] - Formalin fixed paraffin embedded human tonsil stained with RPA using ABC method.
Immunohistochemistry-Paraffin: RPA2 Antibody (9H8) [NB600-565] - Staining of human tonsil

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
9H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RPA2 Antibody (9H8) Summary

Immunogen
Human RPA protein.
Localization
Nuclear
Specificity
This reacts with a 32-34 kDa protein known as Replication Protein A (RPA).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RPA2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation
Application Notes
For IHC: Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Immunocytochemistry/Immunofluorescence was reported in scientific literature. Use in Western blot reported in scientific literature (PMID 24682826). Use in immunprecipitation reported in scientific literature (PMID 27797818).
Positive Control
Tonsil Lysate (NB820-59272)
Publications
Read Publications using
NB600-565 in the following applications:

  • 4 publications
  • IP
    1 publication
  • WB
    3 publications

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
Sodium Azide
Purity
Protein A or G purified

Alternate Names for RPA2 Antibody (9H8)

  • REPA2
  • Replication factor A protein 2
  • replication protein A 32 kDa subunit
  • Replication protein A 34 kDa subunit
  • replication protein A2 (32kD)
  • replication protein A2, 32kDa
  • RF-A protein 2
  • RP-A p32
  • RP-A p34
  • RPA2
  • RPA32
  • RPA34

Background

RPA is involved in DNA replication, repair and recombination. Human RPA is a stable heterotrimer of 70 kD, 32-34 kD and 11-14 kD subunits (RPA70, RPA32 and RPA14 respectively). RPA is required for the SV40 large tumor antigen-catalyzed unwinding for SV40 DNA and stimulates DNA polymerase alpha and delta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for RPA2 Antibody (NB600-565)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(4)
IP
(1)
WB
(3)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB600-565 Applications Species
Piwko W, Mlejnkova LJ, Mutreja K et al. The MMS22L-TONSL heterodimer directly promotes RAD51-dependent recombination upon replication stress. EMBO J. Oct 26 2016 [PMID: 27797818] (ICC/IF, WB, IP, Human) ICC/IF, WB, IP Human
Choi YE, Meghani K, Brault ME et al. Platinum and PARP Inhibitor Resistance Due to Overexpression of MicroRNA-622 in BRCA1-Mutant Ovarian Cancer. Cell Rep. 2016 Jan 26 [PMID: 26774475] (ICC/IF, Human)

Details:
RPA2 antibody (clone 9H8) was used for ICC/IF analysis of RPE1 Fucci cells which were transfected with control ANT or miR-622 ANT and irradiated with 5 Gy IR for 4 hours (Figure 5).
ICC/IF Human
Jiang Q, Paramasivam M, Aressy B et al. MERIT40 cooperates with BRCA2 to resolve DNA interstrand cross-links. Genes Dev 2015 Sep 15 [PMID: 26338419] (WB) WB
Xu Y, Wu X, Her C. hMSH5 Facilitates the Repair of Camptothecin-induced Double-strand Breaks through an Interaction with FANCJ. J Biol Chem 2015 Jul 24 [PMID: 26055704] (Human) Human
Cho NW, Dilley RL, Lampson MA, Greenberg RA. Interchromosomal Homology Searches Drive Directional ALT Telomere Movement and Synapsis. Cell. 2014 Sep 25 [PMID: 25259924]
Shahar OD, Kalousi A, Eini L et al. A high-throughput chemical screen with FDA approved drugs reveals that the antihypertensive drug Spironolactone impairs cancer cell survival by inhibiting homology directed repair. Nucleic Acids Res. 3/25/2014 [PMID: 24682826] (WB, ICC/IF, Human) WB, ICC/IF Human
Tang J, Cho NW, Cui G et al. Acetylation limits 53BP1 association with damaged chromatin to promote homologous recombination. Nat Struct Mol Biol 2013 Feb 3 [PMID: 23377543] (ICC/IF, Human) ICC/IF Human
Coleman KA, Greenberg RA. The BRCA1-RAP80 complex regulates DNA repair mechanism utilization by restricting end resection. J Biol Chem [21335604]. 2011 Feb 18. [PMID: 21335604]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RPA2 Antibody (NB600-565) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

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Bioinformatics

Gene Symbol RPA2
Entrez
Uniprot