ROR alpha/NR1F1 Antibody (784652) [Phycoerythrin]

Th17 differentiated human peripheral blood mononuclear cells (PBMCs, panel A) or resting PBMCs (panel B) were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A) and Mouse Anti-Human RORa/NR1F1 PE-conjugated Monoclonal Antibody (Catalog # IC8924P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Jurkat cells were stained with Mouse Anti-Human ROR alpha /NR1F1 PE‑conjugated Monoclonal Antibody (Catalog # IC8924P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 784652
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

ROR alpha/NR1F1 Antibody (784652) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human ROR alpha /NR1F1
  Arg47-His163
  Accession # P35398
• Specificity: Detects human ROR alpha /NR1F1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RORA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROR alpha/NR1F1 Antibody (784652) [Phycoerythrin]

• DKFZp686M2414
• MGC119326
• NR1F1
• NR1F1MGC119329
• nuclear receptor ROR-alpha
• Nuclear receptor RZR-alpha
• Nuclear receptor subfamily 1 group F member 1
• RAR-related orphan receptor A
• RAR-related orphan receptor alpha
• retinoic acid receptor-related orphan receptor alpha
• retinoid-related orphan receptor alpha
• Retinoid-related orphan receptor-alpha
• ROR alpha
• ROR1
• ROR2
• ROR3
• RORA
• RZRA
• RZR-ALPHA
• RZRAROR-alpha
• transcription factor RZR-alpha

Background

RORa (Retinoid-related Orphan Receptor alpha ) is a member of the NR1 nuclear hormone receptor family. ROR alpha is a DNA binding transcription factor, and can bind as a monomer or homodimer to hormone response elements to enhance expression.  Knockout mice implicate ROR alpha as being essential in the development of the cerebellum. It has also been reported to regulate lymphocyte development and play a role in Th17 differentiation. Human ROR alpha shares 99% amino sequence identity with mouse ROR alpha .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ROR alpha/NR1F1 Antibody (IC8924P)(2)

Publications using IC8924P

• Denise Eckert, Felicitas Rapp, Ayele Taddese Tsedeke, Daniela Kraft, Isabell Wente, Jessica Molendowska et al. Modulation of Differentiation and Bone Resorbing Activity of Human (Pre-) Osteoclasts After X-Ray Exposure Frontiers in Immunology 5/4/2022 [PMID: 35603191]
• T Chang, C Niu, C Sun, Y Ma, R Guo, Z Ruan, Y Gao, X Lu, H Li, Y Lin, J Lin, Z Li Melatonin exerts immunoregulatory effects by balancing peripheral effector and regulatory T helper cells in myasthenia gravis Aging (Albany NY), 2020-11-02;12(0):. 2020-11-02 [PMID: 33136553] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: RORA
• Uniprot:
  • P35398()