Roquin Overexpression Lysate (Native)


Western Blot: Roquin Overexpression Lysate (Adult Normal) [NBP2-05190] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Roquin.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

Roquin Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_742068. The protein contains a C-terminal DDK tag.
Homo sapiens Roquin, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
125.6 kDa

Lysate Details for Roquin

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Roquin Overexpression Lysate (Native)

  • KIAA2025RP5-1198E17.5
  • RING finger and C3H zinc finger protein 1
  • ring finger and CCCH-type domains 1
  • RING finger protein 198
  • RNF198ring finger and CCCH-type zinc finger domains 1
  • roquin


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Contact Information

Product PDFs


Gene Symbol RC3H1

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