RNPS1 Overexpression Lysate (Native)

Images

 
Western Blot: RNPS1 Overexpression Lysate (Adult Normal) [NBL1-15471] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RNPS1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RNPS1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC217673 and based on accession number NM_006711. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens RNA binding protein S1, serine-rich domain (RNPS1), transcript variant 1, mRNA.
Gene
RNPS1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for RNPS1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RNPS1 Overexpression Lysate (Native)

  • E5.1
  • LDC2
  • MGC117332
  • RNA binding protein S1, serine-rich domain
  • RNA-binding protein S1, serine-rich domain
  • RNA-binding protein with serine-rich domain 1
  • SR protein
  • SR-related protein LDC2

Background

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. This protein contains many serine residues. Two splice variants have been found for this gene; both variants encode the same protein. Transcript Variant: This variant (1) contains a different 5' UTR than variant 2 and is the longer transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for RNPS1 Lysate (NBL1-15471) (0)

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Bioinformatics

Gene Symbol RNPS1