RNF8 Overexpression Lysate (Native)

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Western Blot: RNF8 Overexpression Lysate (Adult Normal) [NBL1-15460] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RNF8.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RNF8 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201402 and based on accession number NM_003958. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ring finger protein 8 (RNF8), transcript variant 1, mRNA.
Gene
RNF8

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
55.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RNF8 Overexpression Lysate (Native)

  • ActA Binding Protein 2
  • C3HC4-type zinc finger protein
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ12013
  • KIAA0646UBC13/UEV-interacting ring finger protein
  • ring finger protein (C3HC4 type) 8
  • ring finger protein 8E3 ubiquitin-protein ligase RNF8
  • RNF8

Background

The protein encoded by this gene contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for RNF8 Lysate (NBL1-15460) (0)

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The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

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Bioinformatics

Gene Symbol RNF8
Entrez