RNF39 Antibody

Western Blot: RNF39 Antibody [NB300-842] - (1ug/ml) of human brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Rt, Po
• Applications: WB, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RNF39 Antibody Summary

• Immunogen: Peptide with sequence CDPRAPLRIVPAES corresponding to C-Terminus according to NP_079512.1, NP_739575.1.
• Specificity: This antibody is expected to recognize 2 of the 3 reported isoforms (NP_079512.1 and NP_739575.1 but not NP_739576.1)
• Predicted Species: Rat (100%), Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RNF39
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in human brain lysates (predicted MW of 41 kDa band and 48 kDa band according to NP_079512.1 and NP_739575.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RNF39 Antibody

• HZF
• HZFWLTP (long-term potentiation) induced RING finger protein
• LIRFHZFw1
• Protein HZFw
• ring finger protein 39

Background

RNF39 lies within the major histocompatibility complex class I region on chromosome 6. Studies of a similar rat protein suggest that this gene encodes a protein that plays a role in an early phase of synaptic plasticity. Alternative splicing results in three transcript variants encoding different isoforms. May play a role in prolonged long term-potentiation (LTP) maintenance. Contains 1 RING-type zinc finger.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNF39 Antibody (NB300-842)(1)

Publication using NB300-842

• Matsuo R, Asada A, Fujitani K, Inokuchi K. LIRF, a gene induced during hippocampal long-term potentiation as an immediate-early gene, encodes a novel RING finger protein. Biochem Biophys Res Commun 2001-11-30 [PMID: 11716498]

Bioinformatics

• Gene Symbol: RNF39
• Entrez:
  • 80352(Human)
  • 171387(Rat)
• Uniprot:
  • NP_079512(Human)