RNF139 Antibody

Images

 
Western Blot: RNF139 Antibody [NBP1-23027] - (1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: RNF139 Antibody [NBP1-23027] - (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

RNF139 Antibody Summary

Immunogen
Peptide with sequence C-HTGAAAEEFNDDT corresponding to C-Terminus according to NP_009149.2.
Epitope
internal region
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RNF139
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry 3 - 4 ug/ml
  • Immunohistochemistry-Paraffin 3 - 4 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 75 kDa band observed in human skeletal muscle lysates (calculated MW of 76.0 kDa band according to NP_009149.2). IHC-P: Human placenta shows membranous staining in the cytotrophoblasts.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RNF139 Antibody

  • EC 6.3.2.-
  • HRCA1
  • patched related protein translocated in renal cancer
  • RCA1multiple membrane spanning receptor TRC8
  • ring finger protein 139MGC31961
  • Translocation in renal carcinoma on chromosome 8 protein
  • TRC8E3 ubiquitin-protein ligase RNF139

Background

The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RNF139
Entrez
Uniprot