RNASEH2A Antibody (3G5-F5) - Azide and BSA Free

Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01] - RNASEH2A monoclonal antibody (M01), clone 3G5-F5 Analysis of RNASEH2A expression in Jurkat.

Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01] - Analysis of RNASEH2A over-expressed 293 cell line, cotransfected with RNASEH2A Validated Chimera RNAi ( Cat # H00010535-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNASEH2A monoclonal antibody (M01), clone 3G5-F5 (Cat # H00010535-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01] - Analysis of RNASEH2A expression in transfected 293T cell line by RNASEH2A monoclonal antibody (M01), clone 3G5-F5.Lane 1: RNASEH2A transfected lysate(33.4 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: RNASEH2A Antibody (3G5-F5) [H00010535-M01] - Detection limit for recombinant GST tagged RNASEH2A is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 3G5-F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RNASEH2A Antibody (3G5-F5) - Azide and BSA Free Summary

• Immunogen: RNASEH2A (AAH11748, 1 a.a. ~ 299 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDLSELERDNTGRCRLSSPVPAVCRKEPCVLGVDEAGRGPVLGPMVYAICYCPLPRLADLEALKVADSKTLLESERERLFAKMEDTDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTATGLIQYALDQGVNVTQVFVDTVGMPETYQARLQQSFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKKWQFVEKLQDLDTDYGSGYPNDPKTKAWLKEHVEPVFGFPQFVRFSWRTAQTILEKEAEDVIWEDSASENQEGLRKITSYFLNEGSQARPRSSHRYFLERGLESATSL
• Localization: Nuclear
• Specificity: RNASEH2A - ribonuclease H2, large subunit
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RNASEH2A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA and RNAi Validation.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RNASEH2A Antibody (3G5-F5) - Azide and BSA Free

• AGS4RNase H2 subunit A
• Aicardi-Goutieres syndrome 4 protein
• Aicardi-Goutieres syndrome 4
• JUNB
• ribonuclease H2 subunit A
• ribonuclease H2, large subunit
• ribonuclease H2, subunit A
• Ribonuclease HI large subunit
• Ribonuclease HI subunit A
• ribonuclease HI, large subunit
• RNase H(35)
• RNASEHIEC 3.1.26.4
• RNHIARNase HI large subunit
• RNHL

Background

Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNASEH2A Antibody (H00010535-M01)(1)

Publication using H00010535-M01

• Nozawa K, Doe K, Uomori K et al. Antiribonuclease H2 antibodies are an immune biomarker for systemic lupus erythematosus. Autoimmunity 2017-05-27 [PMID: 28553742]

Bioinformatics

• Gene Symbol: RNASEH2A
• Entrez:
  • 10535(Human)
• OMIM:
  • 606034(Human)
• Uniprot:
  • AAH11748(Human)