RKIP/PBP Antibody

Western blot shows lysates of HUVEC human umbilical vein endothelial cells and A172 human glioblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat RKIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4538) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RKIP at approximately 23 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

RKIP/PBP Antibody Summary

• Immunogen: E. coli-derived recombinant human RKIP
  Met1-Lys187
  Accession # P30086
• Specificity: Detects endogenous human, mouse, and rat RKIP in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PEBP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RKIP/PBP Antibody

• HCNP
• HCNPpp
• hippocampal cholinergic neurostimulating peptide
• Neuropolypeptide h3
• PBP
• PBPPEBP-1
• PEBP1
• PEBP-1
• PEBPHCNPpp
• phosphatidylethanolamine binding protein 1
• phosphatidylethanolamine-binding protein 1
• prostatic binding protein
• Prostatic-binding protein
• Raf kinase inhibitory protein
• RKIP
• RKIPRaf kinase inhibitor protein

Background

Raf kinase inhibitor protein (RKIP) is a member of the phosphatidylethanolamine-binding protein (PEBP) family, and modulates the activation of several signal transduction cascades. By binding the MAP3K Raf-1, unphosphorylated RKIP inhibits activation of the MAP2K MEK. PKC-phosphorylated RKIP dissociates from Raf‑1 and inhibits GRK2, resulting in sustained G-protein signaling. Expressed in many tissues, RKIP is implicated in the regulation of such physiological processes as membrane biosynthesis, spermatogenesis, neural development, and metastasis suppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PEBP1
• Uniprot:
  • P30086(Human)