RKIP/PBP Antibody (1D3-1B6) - Azide and BSA Free

Western Blot: RKIP/PBP Antibody (1D3-1B6) [H00005037-M02] - PBP monoclonal antibody (M02), clone 1D3-1B6 Analysis of PBP expression in HL-60.

Sandwich ELISA: RKIP/PBP Antibody (1D3-1B6) [H00005037-M02] - Detection limit for recombinant GST tagged PBP is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1D3-1B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RKIP/PBP Antibody (1D3-1B6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PBP (AAH31102, 1 a.a. ~ 187 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPVDLSKWSGPLSLQEVDEQPQHPLHVTYAGAAVDELGKVLTPTQVKNRPTSISWDGLDSGKLYTLVLTDPDAPSRKDPKYREWHHFLVVNMKGNDISSGTVLSDYVGSGPPKGTGLHRYVWLVYEQDRPLKCDEPILSNRSGDHRGKFKVASFRKKYELRAPVAGTCYQAEWDDYVPKLYEQLSGK
• Localization: Cytoplasmic
• Specificity: PBP - prostatic binding protein
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PEBP1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RKIP/PBP Antibody (1D3-1B6) - Azide and BSA Free

• HCNP
• HCNPpp
• hippocampal cholinergic neurostimulating peptide
• Neuropolypeptide h3
• PBP
• PBPPEBP-1
• PEBP1
• PEBP-1
• PEBPHCNPpp
• phosphatidylethanolamine binding protein 1
• phosphatidylethanolamine-binding protein 1
• prostatic binding protein
• Prostatic-binding protein
• Raf kinase inhibitory protein
• RKIP
• RKIPRaf kinase inhibitor protein

Background

PBP, also known as RKIP (RAF kinase inhibitor protein), binds ATP, opioids and phosphatidylethanolamine. PBP is able to regulate Aurora kinase B expression and the spindle checkpoint through interaction with the RAF1/MEK/ERK cascade. PBP is able to bind to RAF1 and MEK and competitively disrupt the interaction between these proteins. PBP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. It increases the production of choline acetyltransferase but not acetylcholinesterase. PBP has been implicated as a suppressor of metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PEBP1
• Entrez:
  • 5037(Human)
• OMIM:
  • 604591(Human)
• Uniprot:
  • AAH31102(Human)