RIPX Antibody

Western Blot: RIPX Antibody [NBP2-20168] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: RIPX Antibody [NBP2-20168] - Paraffin-embedded mouse lung. RIPX antibody [N1C1] diluted at 1:500.

Western Blot: RIPX Antibody [NBP2-20168] - Sample (30 ug of whole cell lysate) A: IMR32 7. 5% SDS PAGE gel, diluted at 1:3000.

Immunohistochemistry-Paraffin: RIPX Antibody [NBP2-20168] - Immunohistochemical analysis of paraffin-embedded U251 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu, Rt, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RIPX Antibody Summary

• Description: Novus Biologicals Rabbit RIPX Antibody (NBP2-20168) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human RIPX. The exact sequence is proprietary.
• Localization: Cell projection (By similarity)
• Predicted Species: Rat (99%), Xenopus (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RUFY3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (94%), Bovine (80%), Zebrafish (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RIPX Antibody

• KIAA0871SINGAR1
• protein RUFY3
• rap2 interacting protein x
• Rap2-interacting protein x
• RIPX
• RUN and FYVE domain containing 3
• singar
• Singar1
• Single axon-regulated protein
• single axon-related 1

Background

Implicated in the formation of a single axon by developing neurons. May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RUFY3
• Uniprot:
  • Q7L099(Human)