![Western Blot: RIPPLY2 Antibody (OTI1B5) - Azide and BSA Free [NBP2-73913] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPPLY2.](http://images.novusbio.com/fullsize/RIPPLY2-Antibody-OTI1B5-Azide-and-BSA-Free-Western-Blot-NBP2-73913-img0001.jpg "Western Blot: RIPPLY2 Antibody (OTI1B5) - Azide and BSA Free [NBP2-73913] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPPLY2.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, CyTOF-ready |
| Clone | OTI1B5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human RIPPLY2 (NP_001009994) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RIPPLY2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 13.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
![Western Blot beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg)
![Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Chromatin_Immunoprecipitation_11251.jpg)
![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RIPPLY2 |
![Flow Cytometry: RIPPLY2 Antibody (OTI1B5) - Azide and BSA Free [NBP2-73913] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by RIPPLY2 antibody.](http://images.novusbio.com/fullsize/RIPPLY2-Antibody-OTI1B5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73913-img0003.jpg "Flow Cytometry: RIPPLY2 Antibody (OTI1B5) - Azide and BSA Free [NBP2-73913] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by RIPPLY2 antibody.")
![Flow Cytometry: RIPPLY2 Antibody (OTI1B5) - Azide and BSA Free [NBP2-73913] - Analysis of Jurkat cells, using RIPPLY2 antibody,(Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/RIPPLY2-Antibody-OTI1B5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73913-img0002.jpg "Flow Cytometry: RIPPLY2 Antibody (OTI1B5) - Azide and BSA Free [NBP2-73913] - Analysis of Jurkat cells, using RIPPLY2 antibody,(Red), compared to a nonspecific negative control antibody (Blue).")
![Western Blot TBX6 Antibody [Unconjugated]](https://images.novusbio.com/images2/TBX6_AF4744_Western_Blot_5538.jpg)
![Immunocytochemistry TBX6 Antibody [Unconjugated]](https://images.novusbio.com/images2/TBX6_AF4744_Immunocytochemistry__Immunofluorescence_21853.jpg)
![Immunohistochemistry TBX6 Antibody [Unconjugated]](https://images.novusbio.com/images2/TBX6_AF4744_Immunohistochemistry_6843.jpg)
![SDS-PAGE FGF-8 [Unconjugated]](https://images.novusbio.com/images2/FGF-8_423-F8_332.jpg)
![Bioactivity FGF-8 [Unconjugated]](https://images.novusbio.com/images2/FGF8_423F8_1438.jpg)
![Cell Culture FGF-8 [Unconjugated]](https://images.novusbio.com/images/423-f8_recombinant-human-mouse-fgf-8b-protein-cell-culture-1052024145159..jpg)
![Bioactivity Wnt-3a [Unconjugated]](https://images.novusbio.com/images2/Wnt3a_5036WN_2880.jpg)
![Bioactivity Wnt-3a [Unconjugated]](https://images.novusbio.com/images2/5036-wn_recombinant-human-wnt-3a-protein-bioactivity-3112023115329.jpg)
![Cell Culture Wnt-3a [Unconjugated]](https://images.novusbio.com/images/5036-wn_recombinant-human-wnt-3a-protein-bioactivity-252023103514.jpg)
![Simple Western Pax6 Antibody [Unconjugated]](https://images.novusbio.com/images2/Pax6_AF8150_Simple_Western_17126.jpg)
![Immunocytochemistry Pax6 Antibody [Unconjugated]](https://images.novusbio.com/images2/Pax6_AF8150_Immunocytochemistry_14427.jpg)
![Western Blot Pax6 Antibody [Unconjugated]](https://images.novusbio.com/images2/Pax6_AF8150_Western_Blot_14743.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")