RIPK1/RIP1 [p Ser166] Antibody

Western Blot: RIPK1/RIP1 [p Ser166] Antibody [NBP3-06877] - Analysis of extracts of NIH/3T3 cells, using Phospho-RIPK1-S166 antibody at 1:1000 dilution.NIH/3T3 cells were treated by TNF-alpha (20 ng/mL) at 37c for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.

Product Discontinued

RIPK1/RIP1 [p Ser166] Antibody Summary

• Immunogen: A synthetic phosphorylated peptide around S166 of mouse RIPK1/RIP1 (NP_033094.3).
• Modification: p Ser166
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RIPK1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH 7.3
• Preservative: 0.02% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for RIPK1/RIP1 [p Ser166] Antibody

• Cell death protein RIP
• EC 2.7.11.1
• receptor (TNFRSF)-interacting serine-threonine kinase 1
• receptor interacting protein
• Receptor-interacting protein 1
• receptor-interacting serine/threonine-protein kinase 1
• RIP
• Rip1 kinase
• RIP1
• RIP-1
• RIPFLJ39204
• RIPK1
• Serine/threonine-protein kinase RIP

Background

RIP (Receptor Interacting Protein) is a 74 kD Ser/Thr kinase which interacts with CD95 (Fas/APO1) receptor and the tumor necrosis factor receptor (TNFR1). It is a cell death domain adapter protein which can bind to the adapter proteins TRADD, RAID (CRADD) and TRAF2. RIP contains an N terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C terminal region containing an intracellular death domain motif. RIP activates both p38 MAP Kinase and SAPK families. In vitro, RIP induces apoptosis, as well as SAPK/JNK and NF-kB activation. NF-kB activation through TRADD, TRAF2 and RIP can be triggered also by DR3/APO3 upon activation with APO3/Tweak ligand. DR4 and DR5 also use FADD, TRADD, and RIP in their signal transduction pathways. RIP deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age. RIP possesses kinase activity as it autophosphorylates itself on serine and threonine residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RIPK1