RIPK1/RIP1 Antibody (334640) [Alexa Fluor® 532]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, KO
• Clone: 334640
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

RIPK1/RIP1 Antibody (334640) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human RIPK1/RIP1
  Met1-Asn671
  Accession # Q13546
• Specificity: Detects human, mouse and rat RIPK1/RIP1 in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RIPK1/RIP1 Antibody (334640) [Alexa Fluor® 532]

• Cell death protein RIP
• EC 2.7.11.1
• receptor (TNFRSF)-interacting serine-threonine kinase 1
• receptor interacting protein
• Receptor-interacting protein 1
• receptor-interacting serine/threonine-protein kinase 1
• RIP
• Rip1 kinase
• RIP1
• RIP-1
• RIPFLJ39204
• RIPK1
• Serine/threonine-protein kinase RIP

Background

Receptor-Interacting Protein 1 (RIP1, also known as RIPK1) is a 671 amino acid (aa) 75 kDa protein that contains an N-terminal protein kinase domain, a C-terminal death domain, and a unique internal region called the intermediate domain. RIP1 is a serine/threonine protein kinase and is constitutively expressed in many tissues. RIP1 interacts with the cytoplasmic death domain of FAS and TNF receptors and is an important element in the signal transduction machinery that mediates apoptosis. RIP1 has been shown to interact with a number of proteins including TRADD, TRAF1, TRAF2, and TRAF3, to form larger signaling complexes. These complexes, in turn, activate specific signaling cascades, such as NF kappa B. RIP1 also interacts through the C-terminal RIP homotypic interaction motif (RHIM) of TRIF in TLR3 dependent activation of NF kappa B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.