RIOK2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: RIOK2 Antibody [NBP1-83773] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Antibody staining is shown in green.

Staining of human placenta shows strong membranous positivity in trophoblastic cells.

Staining of human kidney shows moderate membranous positivity in a subset of cells in tubules.

Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.

Staining of human cerebral cortex shows strong cytoplasmic positivity in endothelial cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RIOK2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RIOK2 Antibody - BSA Free (NBP1-83773) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: FPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEFSFSDGEVAEKAEVYRSENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RIOK2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  RIOK2 Protein (NBP1-83773PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RIOK2 Antibody - BSA Free

• EC 2.7.11.1
• FLJ11159
• RIO kinase 2 (yeast)
• RIO kinase 2
• RIO2
• RIOK2
• serine/threonine-protein kinase RIO2

Background

RIO kinase 2 (RIOK2) is a member of the RIO family of atypical serine protein kinases first characterized in yeast. There are three RIO kinase subfamilies: RIO1, RIO2, and RIO3. RIOK1 and RIOK2 proteins are present in organisms from Archaea to humans. Studies of RIO kinases in yeast indicate that they play a role in ribosome biogenesis. Human RIOK2 has been shown to be a part of a late 40S pre-ribosomal particle and have influence on 40S maturation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RIOK2