Rictor Antibody (1F3) - Azide and BSA Free

Western Blot: Rictor Antibody (1F3) [H00253260-M01] - RICTOR monoclonal antibody (M01), clone 1F3 Analysis of RICTOR expression in Hela S3 NE.

Immunohistochemistry-Paraffin: Rictor Antibody (1F3) [H00253260-M01] - Analysis of monoclonal antibody to RICTOR on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.

Western Blot: Rictor Antibody (1F3) [H00253260-M01] - Analysis of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody (M01), clone 1F3.Lane 1: RICTOR transfected lysate(29.8 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: Rictor Antibody (1F3) [H00253260-M01] - Analysis of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RICTOR MaxPab rabbit polyclonal antibody.

Sandwich ELISA: Rictor Antibody (1F3) [H00253260-M01] - Detection limit for recombinant GST tagged RICTOR is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Func, IHC, IP
• Clone: 1F3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Rictor Antibody (1F3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Rictor Antibody (1F3) - Azide and BSA Free (H00253260-M01) is a monoclonal antibody validated for use in IHC, WB, ELISA and IP. Anti-Rictor Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: RICTOR (NP_689969, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAIGRGRSLKNLRVRGRNDSGEENVPLDLTREPSDNLREILQNVARLQGVSNMRKLGHLNNFTKLLCDIGHSEEKLGFHYEDIIICLRLALLNEAKE
• Specificity: RICTOR - rapamycin-insensitive companion of mTOR
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RICTOR
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rictor Antibody (1F3) - Azide and BSA Free

• AVO3 homolog
• AVO3
• hAVO3
• KIAA1999DKFZp686B11164
• mAVO3
• MGC39830
• PIA
• Pianissimo
• rapamycin-insensitive companion of mTOR
• Rictor
• RPTOR independent companion of MTOR, complex 2
• TORC2-specific protein AVO3

Background

RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rictor Antibody (H00253260-M01)(5)

Publications using H00253260-M01

• Wu X, Schneider N, Platen A et al. In situ characterization of the mTORC1 during adipogenesis of human adult stem cells on chip. Proc Natl Acad Sci U S A 2016-07-05 [PMID: 27382182]
• Populo H, Tavares S, Faustino A et al. GNAQ and BRAF mutations show differential activation of the mTOR pathway in human transformed cells. PeerJ. 2013-07-23 [PMID: 23904987]
• Wang JQ, Chen JH, Chen YC et al. Interaction between NBS1 and the mTOR/Rictor/SIN1 complex through specific domains. PLoS One. 2013-01-01 [PMID: 23762398] (Human)
• Faustino A, Couto JP, Populo H et al. mTOR Pathway Overactivation in BRAF Mutated Papillary Thyroid Carcinoma. J Clin Endocrinol Metab. 2012-05-01 [PMID: 22549934]
• Naimi M, Gautier N, Chaussade C et al. Nuclear forkhead box O1 controls and integrates key signaling pathways in hepatocytes. Endocrinology 2007-05-01 [PMID: 17303659]

Bioinformatics

• Gene Symbol: RICTOR
• Entrez:
  • 253260(Human)
• OMIM:
  • 609022(Human)
• Uniprot:
  • NP_689969(Human)