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Ribosomal protein S27 Antibody (7E3)

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Immunocytochemistry/Immunofluorescence: Ribosomal protein S27 Antibody (7E3) [NBP2-37671] - Confocal immunofluorescence analysis of Hela cells (A), BCBL-1 cells (B) and L1210 cells (C) using MPS1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunocytochemistry/ Immunofluorescence: Ribosomal protein S27 Antibody (7E3) [NBP2-37671] - Confocal immunofluorescence analysis of Hela cells (A), BCBL-1 cells (B) and L1210 cells (C) using MPS1 mouse mAb (green). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, ICC/IF
Clone
7E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Ribosomal protein S27 Antibody (7E3) Summary

Immunogen
Purified recombinant fragment of MPS1 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RPS27
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Ribosomal protein S27 Antibody (7E3)

  • Metallopan-stimulin 1
  • MPS140S ribosomal protein S27
  • MPS-1metallopanstimulin 1
  • ribosomal protein S27 (metallopanstimulin 1)
  • ribosomal protein S27

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAD2L1 antibody NB100-563 (lot 3) used for IP at 3 ug/mg lysate. MAD2L1 was also immunoprecipitated by a previous lot (lot 2) and by rabbit anti-MAD2L1 antibody NB100-564. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Immunohistochemistry-Paraffin: Mxi1 Antibody [NBP1-88626] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.
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Applications: WB, IHC, IHC-P
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Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody diluted by 1:5000.
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Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NBP1-32775] - Paraffin-embedded SW480 Xenograft, using antibody at 1:100 dilution.
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Immunohistochemistry-Paraffin: Mad Antibody [NBP1-89979] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
Immunohistochemistry-Paraffin: Mad Antibody [NBP1-89979] - Staining of human bone marrow shows high expression.
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Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - FFPE section of cerebral cortex of an Alzheimer patient processed with Ubiquitin antibody using HRP/DAB (Brown) and also stained with haemotoxylin (Blue). A typical flame shaped tangle is seen in a pyramidal neuron in the center and is surrounded by some dystrophic neurites, also strongly ubiquitin positive. Both are commonly seen in cortical and hippocampal Alzheimer brain sections and are typical for this disease, but are rare or absent in healthy brain.
Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Western blot of Ubiquitin expression on (Lane 1) poly-ubiquitin (lys63 linked), (Lane 2) pure ubiquitin and crude homogenates of adult rat, (Lane 3) cortex, (Lane 4) cerebellum and (Lane 5) brain stem.
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Western Blot: Ribosomal Protein S29 Antibody [NBP1-57477] - Jurkat cell lysate, concentration 1.25ug/ml.
Immunohistochemistry: Ribosomal Protein S29 Antibody [NBP1-57477] - Human Heart cell Cellular data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X.
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Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hec1 stained by Hec1 antibody [9G3.23] diluted at 1:500. Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500.Blue: Hoechst 33342 staining.
Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - WB analysis of NDC80 in U2OS Whole Cell Lysate. Verified customer review from 1DegreeBio.
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Western Blot: Smad3 Antibody [NB100-56479] - Analysis in human fibroblast and HeLa whole cell lysates. Untreated lysates show a band around 55 Kda. 1:1000 dilution, 1 hour blocking in milk 5%. Image from a verified customer review.
Immunocytochemistry/Immunofluorescence: Smad3 Antibody [NB100-56479] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Smad3 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Smad3 Antibody [NB100-56479] - Human liver tissue probed with SMAD3 antibody (bottom left, right) and isotype control antibody (top left) at 10 ug/ml.
(1 Review)
(2 Publications)
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Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: RPL27 Antibody [NBP1-82856] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry-Paraffin: RPL27 Antibody [NBP1-82856] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Western Blot: RPL27 Antibody [NBP1-82856] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
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MAD3 Antibody
(1 Publication)
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Applications: ICC/IF, IHC, IHC-P, PEP-ELISA

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Bioinformatics

Gene Symbol RPS27